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Single amino acid substitution in Plasmodium yoelii erythrocyte ligand determines its localization and controls parasite virulence


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Title: Single amino acid substitution in Plasmodium yoelii erythrocyte ligand determines its localization and controls parasite virulence
Authors: Otsuki, Hitoshi / Kaneko, Osamu / Thongkukiatkul, Amporn / Tachibana, Mayumi / Iriko, Hideyuki / Takeo, Satoru / Tsuboi, Takafumi / Torii, Motomi
Issue Date: 28-Apr-2009
Publisher: the National Academy of Sciences
Citation: PNAS, 106(17), pp.7167-7172; 2009
Abstract: The major virulence determinant of the rodent malaria parasite, Plasmodium yoelii, has remained unresolved since the discovery of the lethal line in the 1970s. Because virulence in this parasite correlates with the ability to invade different types of erythrocytes, we evaluated the potential role of the parasite erythrocyte binding ligand, PyEBL. We found 1 amino acid substitution in a domain responsible for intracellular trafficking between the lethal and nonlethal parasite lines and, furthermore, that the intracellular localization of PyEBL was distinct between these lines. Genetic modification showed that this substitution was responsible not only for PyEBL localization but also the erythrocyte-type invasion preference of the parasite and subsequently its virulence in mice. This previously unrecognized mechanism for altering an invasion phenotype indicates that subtle alterations of a malaria parasite ligand can dramatically affect host–pathogen interactions and malaria virulence.
Keywords: erythrocyte / invasion / ligand / malaria / Plasmodium yoelii
URI: http://hdl.handle.net/10069/22077
ISSN: 00278424
DOI: 10.1073/pnas.0811313106
PubMed ID: 19346470
Rights: Copyright ©2009 by the National Academy of Sciences
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22077

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