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Single-cell observation of phagocytosis by human blood dendritic cells


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Title: Single-cell observation of phagocytosis by human blood dendritic cells
Authors: Ishimoto, Hiroshi / Yanagihara, Katsunori / Araki, Nobuko / Mukae, Hiroshi / Sakamoto, Noriho / Izumikawa, Koichi / Seki, Masafumi / Miyazaki, Yoshitsugu / Hirakata, Yoichi / Mizuta, Yohei / Yasuda, Kenji / Kohno, Shigeru
Issue Date: Jul-2008
Publisher: National Institute of Infectious Diseases
Citation: Japanese Journal of Infectious Diseases, 61(4), pp. 294-297; 2008
Abstract: Time-lapse video microscopic observation is useful for analysis of cell biology, especially in rapid response of immune cells. Dendritic cells (DCs) have multiple functions in the immune system, and DCs in peripheral blood play an especially important role at the front line of infection. We have developed a time-lapse video microscopic method for the evaluation of single myeloid DCs (MDC-1) from human peripheral blood. MDC-1 displayed generalized plasma membrane ruffling and phagocytosis of Pseudomonas aeruginosa. The morphological changes of MDC-1 increased following stimulation with P. aeruginosa but not after stimulation with supernatant from a P. aeruginosa culture. The activation of these morphological changes in MDC-1 could be quantitatively analyzed using the time-lapse video microscopy. This novel system may be useful for the evaluation of rapid response with human immune cells against bacterial infection.
URI: http://hdl.handle.net/10069/22111
ISSN: 13446304
PubMed ID: 18653972
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22111

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