DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > Faculty of Fisheries > Articles in academic journal >

Comparative analysis of the production of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) from macrophages exposed to high virulent and low virulent strains of Edwardsiella tarda.


File Description SizeFormat
FSI27_386.pdf154.83 kBAdobe PDFView/Open

Title: Comparative analysis of the production of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) from macrophages exposed to high virulent and low virulent strains of Edwardsiella tarda.
Authors: Ishibe, Keiko / Yamanishi, Tomohiro / Wang, Yajun / Osatomi, Kiyoshi / Hara, Kenji / Kanai, Kinya / Yamaguchi, Kenichi / Oda, Tatsuya
Issue Date: Aug-2009
Publisher: Elsevier Ltd
Citation: Fish & shellfish immunology, 27(2), pp.386-389; 2009
Abstract: We previously reported that high virulent strain (NUF251) of Edwardsiella tarda has an ability to prevent the production of reactive oxygen species by macrophages, and is even capable of surviving and multiplying within Japanese flounder (Paralichthys olivaceus) peritoneal macrophages, whereas the low virulent strain (NUF194) has no such ability. In this study, we found that NUF251 and NUF194 induced NO and TNF-alpha production from Japanese flounder peritoneal macrophages, and NUF251 caused faster induction of NO release and much higher level of TNF-alpha production than NUF194. In addition, similar differences between two strains in terms of the induction of NO and TNF-alpha production were also observed in mouse macrophage cell line RAW264.7 cells. Our results suggest that the potent ability to induce the production of NO and TNF-alpha from macrophages may be one of the factors responsible for the virulence of E. tarda.
Keywords: Edwardsiella tarda / Bacterial infection / Japanese flounder / Macrophage / Mouse macrophage cell line / Nitric oxide / Tumor necrosis factor-α
URI: http://hdl.handle.net/10069/22145
ISSN: 10504648
DOI: 10.1016/j.fsi.2009.06.002
PubMed ID: 19539031
Rights: Copyright © 2009 Elsevier Ltd All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22145

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace