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Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen


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Title: Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen
Authors: Bae, Inwoo / Osatomi, Kiyoshi / Yoshida, Asami / Yamaguchi, Atsuko / Tachibana, Katsuyasu / Oda, Tatsuya / Hara, Kenji
Issue Date: May-2009
Citation: Fisheries Science, 75(3), pp.765-770; 2009
Abstract: A translucent collagen gel was formed from a transparent acidic solution of red stingray collagen by adjusting to physiological ionic strength and pH in phosphate buffer and then incubating at 25–37°C. During fibril formation from red stingray collagen, the turbidity increased when the NaCl concentration was increased at constant pH and the rate of fibril formation was accelerated by higher pH or lower NaCl concentration. The T m of red stingray collagen fibrillar gel was estimated as 44.3 ± 3.5°C, which was higher than that of the collagen solution, 33.2°C. In addition, red stingray collagen gel maintained its shape without melting and was suitable for culture of mouse stromal cells at 37°C.
Keywords: Cell culture / Collagen fibrillar gel / Fibril formation / Fish collagen / Red stingray / Type I collagen
URI: http://hdl.handle.net/10069/22155
ISSN: 09199268
DOI: 10.1007/s12562-009-0085-1
Rights: © The Japanese Society of Fisheries Science 2009 / The original publication is available at www.springerlink.com
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22155

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