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Keratinocyte growth factor accelerates compensatory growth in the remaining lung after trilobectomy in rats.


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タイトル: Keratinocyte growth factor accelerates compensatory growth in the remaining lung after trilobectomy in rats.
著者: Matsumoto, Keitaro / Nagayasu, Takeshi / Hishikawa, Yoshitaka / Tagawa, Tsutomu / Yamayoshi, Takatomo / Abo, Takafumi / Tobinaga, Shuichi / Furukawa, Katsuro / Koji, Takehiko
発行日: 2009年 6月
出版者: Mosby
引用: The Journal of thoracic and cardiovascular surgery, 137(6), pp.1499-1507; 2009
抄録: OBJECTIVE: In rats pulmonary resection is followed by lung compensatory growth. However, the molecular mechanism underlying lung compensatory growth remains unclear. Keratinocyte growth factor is expressed in lung tissue and is considered a possible mitogen for lung epithelial cells. The objectives of this study were to define the role of keratinocyte growth factor and its receptor in rat lung compensatory growth after trilobectomy and the effect of exogenous keratinocyte growth factor gene transfection. METHODS: Adult Lewis rats were used. Right trilobectomy was performed in the operation group and sham thoracotomy in the sham group. In the operation group, keratinocyte growth factor-FLAG or FLAG expression vector was transfected directly into the lung by means of electroporation. Expression of keratinocyte growth factor and its receptor and alveolar cell proliferation index based on proliferating cell nuclear antigen levels were measured in the right lung at day 14 after the operation. RESULTS: Proliferating cell nuclear antigen, keratinocyte growth factor, and keratinocyte growth factor receptor expression in lung epithelial cells was significantly increased at day 4 after trilobectomy. Transfection of keratinocyte growth factor-FLAG expression vector resulted in further significant enhancement of proliferating cell nuclear antigen at day 4 after trilobectomy; however, the transfection of FLAG expression vector did not alter the enhancement of proliferating cell nuclear antigen. Exogenous expression of keratinocyte growth factor in the remaining lung by means of electroporation significantly augmented epithelial proliferation and decreased the average airspace distance (mean linear intercept). CONCLUSION: Our results implicate keratinocyte growth factor in the induction of alveolar epithelial cell proliferation for compensatory lung growth and indicate that overexpression of keratinocyte growth factor in the remaining lung by means of electroporation significantly augmented lung epithelial proliferation.
URI: http://hdl.handle.net/10069/22184
ISSN: 00225223
DOI: 10.1016/j.jtcvs.2008.11.037
PubMed ID: 19464471
権利: Copyright © 2009 The American Association for Thoracic Surgery Published by Mosby, Inc.
資料タイプ: Journal Article
原稿種類: author
出現コレクション:030 学術雑誌論文

引用URI : http://hdl.handle.net/10069/22184

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