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Long-term persistence of X-ray-induced genomic instability in quiescent normal human diploid cells.


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タイトル: Long-term persistence of X-ray-induced genomic instability in quiescent normal human diploid cells.
著者: Suzuki, Keiji / Kashino, Genro / Kodama, Seiji / Watanabe, Masami
発行日: 2009年12月 1日
出版者: Elsevier BV
引用: Mutation research - Fundamental and Molecular Mechanisms of Mutagenesis, 671(1-2), pp.33-39; 2009
抄録: Ionizing radiation can induce genomic instability in the progeny of irradiated cells, as was demonstrated in various experimental systems. Most in vitro studies have utilized replicating cells, but it is not clear whether radiation-induced genomic instability persists in quiescent cells. Here we show the induction of X-ray-induced genomic instability in normal human diploid cells irradiated and maintained in a quiescent state for up to 24 months while cells were subcultured approximately once every 2-3 months. Every 12 months, a fraction of the irradiated cell population was stimulated to divide by culturing at a low density, and we found that these cells showed increased frequencies of phosphorylated ATM foci, decreased colony-forming ability, and increased frequency of chromosomal aberrations. No significant increases in ROS levels were detected in long-term cultured cells. These results suggest that there are ROS-independent mechanism(s) induced by radiation, which can generate persistent delayed effects in quiescent cells, and could ultimately contribute to carcinogenesis.
キーワード: Chromosome / DNA damage / In vitro / Instability / Ionizing radiation
URI: http://hdl.handle.net/10069/22231
ISSN: 00275107
DOI: 10.1016/j.mrfmmm.2009.08.010
PubMed ID: 19712688
権利: Copyright © 2009 Elsevier B.V. All rights reserved.
資料タイプ: Journal Article
原稿種類: author
出現コレクション:110 学術雑誌論文

引用URI : http://hdl.handle.net/10069/22231

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