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Cathepsin L is required for ecotropic murine leukemia virus infection in NIH3T3 cells.


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タイトル: Cathepsin L is required for ecotropic murine leukemia virus infection in NIH3T3 cells.
著者: Yoshii, Hiroaki / Kamiyama, Haruka / Minematsu, Kazuo / Goto, Kensuke / Mizota, Tsutomu / Oishi, Kazunori / Katunuma, Nobuhiko / Yamamoto, Naoki / Kubo, Yoshinao
発行日: 2009年11月25日
出版者: Elsevier
引用: Virology, 394(2), pp.227-234; 2009
抄録: Recently it has been reported that a cathepsin B inhibitor, CA-074Me, attenuates ecotropic murine leukemia virus (Eco-MLV) infection in NIH3T3 cells, suggesting that cathepsin B is required for the Eco-MLV infection. However, cathepsin B activity was negative or extremely low in NIH3T3 cells. How did CA-074Me attenuate the Eco-MLV infection? The CA-074Me treatment of NIH3T3 cells inhibited cathepsin L activity, and a cathepsin L specific inhibitor, CLIK148, attenuated the Eco-MLV vector infection. These results indicate that the suppression of cathepsin L activity by CA-074Me induces the inhibition of Eco-MLV infection, suggesting that cathepsin L is required for the Eco-MLV infection in NIH3T3 cells. The CA-074Me treatment inhibited the Eco-MLV infection in human cells expressing the exogenous mouse ecotropic receptor and endogenous cathepsins B and L, but the CLIK148 treatment did not, showing that only the cathepsin L suppression by CLIK148 is not enough to prevent the Eco-MLV infection in cells expressing both of cathepsins B and L, and CA-074Me inhibits the Eco-MLV infection by suppressing both of cathepsins B and L. These results suggest that either cathepsin B or L is sufficient for the Eco-MLV infection.
キーワード: Cathepsin B / Cathepsin L / Ecotropic murine leukemia virus
URI: http://hdl.handle.net/10069/22253
ISSN: 00426822
DOI: 10.1016/j.virol.2009.08.045
PubMed ID: 19781728
権利: Copyright © 2009 Elsevier Inc. All rights reserved.
資料タイプ: Journal Article
原稿種類: author
出現コレクション:120 学術雑誌論文

引用URI : http://hdl.handle.net/10069/22253

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