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Rapid identification of penicillin and macrolide resistance genes and simultaneous quantification of Streptococcus pneumoniae in purulent sputum samples by use of a novel real-time multiplex PCR assay.


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Title: Rapid identification of penicillin and macrolide resistance genes and simultaneous quantification of Streptococcus pneumoniae in purulent sputum samples by use of a novel real-time multiplex PCR assay.
Authors: Fukushima, Kazuko Y / Yanagihara, Katsunori / Hirakata, Yoichi / Sugahara, Kazuyuki / Morinaga, Yoshitomo / Kohno, Shigeru / Kamihira, Shimeru
Issue Date: Jul-2008
Publisher: American Society for Microbiology
Citation: Journal of Clinical Microbiology, 46(7), pp.2384-2388; 2008
Abstract: We evaluated a real-time quantitative PCR combined with a multiplex PCR assay for the quantification of Streptococcus pneumoniae and the simultaneous detection of drug-resistant genes by gel-based PCR, using purulent sputum samples. This assay correctly quantified S. pneumoniae and identified their penicillin and erythromycin susceptibilities directly from samples within 3 h.
URI: http://hdl.handle.net/10069/22255
ISSN: 1098660X
DOI: 10.1128/JCM.00051-08
PubMed ID: 18463207
Rights: Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22255

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