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Simvastatin suppresses the differentiation of C2C12 myoblast cells via a Rac pathway.


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Title: Simvastatin suppresses the differentiation of C2C12 myoblast cells via a Rac pathway.
Authors: Baba, Tomomi T / Nemoto, Takayuki K / Miyazaki, Toshihiro / Oida, Shinichiro
Issue Date: May-2008
Publisher: Springer Netherlands
Citation: Journal of muscle research and cell motility, 29(2-5), pp.127-134; 2008
Abstract: Statins, which are known as cholesterol-lowering drugs, have several additional effects including the enhancement of bone formation and the stimulation of smooth muscle cell proliferation. In this study, we investigated the signal pathway of simvastatin operating in C2C12 myoblast cells. Myotube formation of C2C12 cells was efficiently blocked by 1 muM simvastatin, and mevalonic acid was able to cancel this effect. Geranylgeranyl pyrophosphate restored the myotube formation, whereas farnesyl pyrophosphate did not. These findings demonstrate that the Rho family, such as Rho, Rac and Cdc42, occurring downstream of geranylgeranyl pyrophosphate in the mevalonic acid pathway, was involved in the simvastatin-mediated blockage of myotube formation. An inhibitor of Rho kinase did not influence the myotube formation; whereas an inhibitor of Rac blocked this process. Taken together, we conclude that the differentiation of C2C12 cells into myotubes was blocked by simvastatin through the pathway mediated by Rac, not by Rho.
Keywords: C2C12 / Cholesterol-lowering drugs / Myoblast / Myogenin / Myotube / Statins
URI: http://hdl.handle.net/10069/22301
ISSN: 01424319
DOI: 10.1007/s10974-008-9146-9
PubMed ID: 18792797
Rights: © Springer Science+Business Media B.V. 2008 / The original publication is available at www.springerlink.com
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22301

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