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ENZYME HISTOCHEMISTRY OF BRUGIA PAHANGI 1. Localization of Acid Phosphatase Activity in Developing Larvae in Mosquito (Aedes aegypti)


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Title: ENZYME HISTOCHEMISTRY OF BRUGIA PAHANGI 1. Localization of Acid Phosphatase Activity in Developing Larvae in Mosquito (Aedes aegypti)
Other Titles: Brugia pahangiの酵素組織化学 1.蚊(Aedes aegypti)内発育幼虫における酸性フォスファターゼ活性の局在
Authors: Kimura, Eisaku / Nakajima, Yasuo / Aoki, Yoshiki
Authors (alternative): 木村, 英作 / 中島, 康雄 / 青木, 克己
Issue Date: 15-Mar-1983
Publisher: 日本熱帯医学会 / Japanese Society of Tropical Medicine
Citation: 日本熱帯医学会雑誌, vol.11(1), pp.7-15; 1983
Abstract: イヌ末梢血中ならびにスナネズミ腹腔中のミクロフィラリアと,それらの蚊体内で発育中の幼虫について,酸性フォスファターゼの局在を組織化学的に比較した。ミクロフィラリアは全体が赤く染まり, excretory vesicleとanal vesicleが暗赤色を呈する。イヌのミクロフィラリアでは鞘は陰性であるのに対し,スナネズミのものは大部分が陽性である。イヌを吸血した蚊とは対照的に,スナネズミのミクロフィラリアを摂取した蚊では, 最初の2日聞に頻回に染色されない幼虫を認める。3日日以降,陽性の発育中の腸管を見る。l回目の脱皮後に, 食道は赤色の複線として見える。7日目に,食道,腸管,発育中の虹門, amphid は強い活性を示すが, 神経輪は陰性である。感染幼虫の角皮下層と肛門に陽性反応を認める。 / Histochemical localization of acid phosphatase was reported on the microfilariae isolated from the peripheral blood of the dog and their developing larvae in mosquitoes in comparison with the localization in those from the jird's peritoneal cavity and their larvae. Whole body of microfilariae stained red with dark red excretory vesicle and anal vesicle. The sheaths were negative in those from the dog, while positive in most microfilariae from the jird. During the first two days after the intake unstained larvae were frequently found in the mosquitoes fed jird microfilariae in contrast to those fed on the dog. From the third day on, positive developing intestine was observed. After the first molt the esophagus appeared as a red double line. On the 7th day strong activity was found at the esophagus, intestine, developing anus and amphids, while the nerve ring was negative. Positive reaction was detected at the hypodermis and anus of infective larvae.
URI: http://hdl.handle.net/10069/22378
ISSN: 03042146
Rights: Japanese Society of Tropical Medicine
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22378

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