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APPLICATION OF TWO TYPES OF TRYPANOSOMA CRUZI AMASTIGOTES OF DIFFERENT VIRULENCE TO ELISA FOR CHAGAS' DISEASE


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Title: APPLICATION OF TWO TYPES OF TRYPANOSOMA CRUZI AMASTIGOTES OF DIFFERENT VIRULENCE TO ELISA FOR CHAGAS' DISEASE
Other Titles: Trypanosoma cruziから得た2種のAmastigoteを抗原として用いたELISA法の試み
Authors: Maldonado, Marisel / Ichinose, Yoshio / Samudio, Margarita / Arias, Antonieta De / Sakamoto, Makoto / Axorero, Ricardo Moreno / Kanbara, Hiroji
Authors (alternative): Maldonado, M. / 一瀬, 休生 / Samudio, M. / Arias, A. de / 坂本, 信 / Azorero, R.M. / 神原, 廣二
Issue Date: 15-Dec-1990
Publisher: 日本熱帯医学会 / Japanese Society of Tropical Medicine
Citation: 日本熱帯医学会雑誌, vol.18(4), pp.325-332; 1990
Abstract: マウス線維芽細胞内で増殖したAmastigote(Am-C) およびcell-freeの液体培地中で増殖したAmastigote(Am-T) を固相抗原としたELISA法を用いて,患者血清に対する反応性の検討を行った。AmastigoteはG-1株およびTulahuen株の強毒,弱毒クローンから得られたものを用いた。抗原として用いたAmastigoteは超音波破砕した後,不可溶性部分を含んだままで,マイクロタイタープレートに固相として用いた。血清は1例の急性期患者を含む51例のシャーガス病患者血清1例のシャーガス病との混合感染患者血清を含む10例の皮膚リーシュマニア症患者血清,および3例のシャーガス病,もしくは皮膚リーシュマニア症との混合感染患者を含む10例のトキソプラズマ症患者血清を用いた。その結果,調整法の異なるそれぞれのAmastigoteの間,およびクローン間,株間でもそれぞれにおいてかなり高い相関が見られた。強毒株を固相抗原として用いたELISAにおいて高い反応性が,またcell-freeの液体培地中で増殖したAmastigoteを用いたELISAにおいて高い特異性が見られた。通常のEpimastigoteを用いたELISA法で陰性であった1例の急性期患者血清は,G-1-T, H-23-Tにおいて陽性と判定された。 / Trypanosoma cruzi amastigotes derived from fibroblast cultures and from cell-free liquid medium were comparatively evaluated as solid-phase antigens of ELISA for Chagas' disease. Amastigotes were obtained from the high- and low-virulent clones of the Tulahuen strain and from the G-1 strain. They were sonicated and directly applied into wells of a microplate to use insoluble particles as antigens. Fifty-one sera from patients of Chagas' disease including one acute case (with Romana's sign), 10 sera from patients of cutaneous leishmaniasis including one serum of mixed infection with Chagas' disease and 10 sera from patients of toxoplasmosis including three of mixed infection with either Chagas' disease or leishmaniasis were examined. All amastigotes from different sources and from different clones or strains showed considerable correlation with each other. Slightly higher sensitivity was found in amastigotes of high-virulent clones and slightly higher specificity in those from cell-free culture. One acute case was detected as positive by these methods although it was negative by the usual ELISA using epimastigotes.
URI: http://hdl.handle.net/10069/22393
ISSN: 03042146
Rights: Japanese Society of Tropical Medicine
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22393

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