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Rapid and significant induction of TRAIL-mediated type II cells in apoptosis of primary salivary epithelial cells in primary Sjögren's syndrome.


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Title: Rapid and significant induction of TRAIL-mediated type II cells in apoptosis of primary salivary epithelial cells in primary Sjögren's syndrome.
Authors: Nakamura, Hideki / Kawakami, Atsushi / Iwamoto, Naoki / Ida, Hiroaki / Koji, Takehiko / Eguchi, Katsumi
Issue Date: Nov-2008
Publisher: Springer Netherlands
Citation: Apoptosis, 13(11), pp.1322-1330; 2008
Abstract: Expressions of the effector molecules of Fas-mediated apoptosis in primary cultured salivary gland epithelial cells (SGEC) of primary Sjögren's syndrome (pSS) remain to be clarified. We focused on Fas-mediated caspase cleavage compared to tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-mediated apoptosis. Induction of apoptosis was performed by anti-Fas antibody coupled with PI3K inhibitor, or TRAIL. Activation of caspases, cytochrome C, and apoptotic protease activating factor-1 (Apaf-1) was determined by western blotting or immunofluorescence observed by confocal microscopy. Fas-mediated apoptosis and activation of caspase 3/8 were induced in the presence of LY294002. TRAIL-induced apoptosis in SGEC, which was stronger than that induced by anti-Fas antibody. TRAIL-induced caspase 9 cleavage accompanied by activation of cytochrome C and Apaf-1 were not mediated by anti-Fas antibody. Our results suggest that death receptor-dependent apoptosis in primary cultured SGEC is regulated by the engagement of type II cells in pSS.
Keywords: Caspases / Fas / Sjögren's syndrome / TRAIL / Type II cells
URI: http://hdl.handle.net/10069/22525
ISSN: 13608185
DOI: 10.1007/s10495-008-0261-2
PubMed ID: 18791828
Rights: © Springer Science+Business Media, LLC 2008 / The original publication is available at www.springerlink.com
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22525

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