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Quantitative detection of metallo-beta-lactamase of blaIMP-cluster-producing Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis for rapid diagnosis and treatment of nosocomial infection.


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Title: Quantitative detection of metallo-beta-lactamase of blaIMP-cluster-producing Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis for rapid diagnosis and treatment of nosocomial infection.
Authors: Motoshima, Maiko / Yanagihara, Katsunori / Yamamoto, Kazuko / Morinaga, Yoshitomo / Matsuda, Junichi / Sugahara, Kazuyuki / Hirakata, Yoichi / Yamada, Yasuaki / Kohno, Shigeru / Kamihira, Shimeru
Issue Date: Jun-2008
Publisher: Elsevier Inc.
Citation: Diagnostic microbiology and infectious disease, 61(2), pp.222-226; 2008
Abstract: In this study, we established the rapid quantitative detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa in clinical isolates and samples using real-time polymerase chain reaction (PCR) targeting gyrB (identification of P. aeruginosa) and blaIMP. The relative sensitivities and specificities of this real-time PCR assay were as follows: 100.0% and 100.0% for clinical isolates, and 100.0% and 98.4% for clinical specimens, respectively. The relative sensitivities and specificities of blaIMP-PCR were 100.0% in both clinical isolates and clinical specimens. The present PCR assay was easily and quickly performed, and it accurately detected P. aeruginosa and metallo-beta-lactamase.
Keywords: blaIMP / gyrB / Melting curve analysis / Metallo-β-lactamase / Pseudomonas aeruginosa / Quantitative detection
URI: http://hdl.handle.net/10069/22527
ISSN: 07328893
DOI: 10.1016/j.diagmicrobio.2008.01.018
PubMed ID: 18343622
Rights: Copyright © 2008 Elsevier Inc. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22527

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