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RNAi-mediated down-regulation of alpha-actinin-4 decreases invasion potential in oral squamous cell carcinoma.


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Title: RNAi-mediated down-regulation of alpha-actinin-4 decreases invasion potential in oral squamous cell carcinoma.
Authors: Yamada, Shin-ichi / Yanamoto, Souichi / Yoshida, Hajime / Yoshitomi, Izumi / Kawasaki, Goro / Mizuno, Akio / Nemoto, Takayuki K.
Issue Date: Jan-2010
Publisher: Elsevier Ltd.
Citation: International journal of oral and maxillofacial surgery, 39(1), pp.61-67; 2010
Abstract: alpha-actinin-4, originally identified as an actin-binding protein associated with cell motility, invasion, and metastasis of cancer cells, appears to be overexpressed in various human epithelial carcinomas, including colorectal, breast, esophageal, ovarian, and non-small cell lung carcinomas. The authors evaluated whether alpha-actinin-4 might be appropriate as a molecular target for cancer gene therapy. In 64 primary oral squamous cell carcinomas (OSCCs) and 10 normal oral mucosal specimens, and in seven human OSCC cell lines, alpha-actinin-4 expression was evaluated immunologically and correlations with clinicopathologic factors were examined. Overexpression of alpha-actinin-4 was detected in 38 of 64 oral squamous cell carcinomas (70%); significantly more frequently than in normal oral mucosa. The expression of alpha-actinin-4 was significantly associated with invasion potential defined by the Matrigel invasion assay. Cancer cell lines with higher alpha-actinin-4 expression had greater invasive potential. An RNAi-mediated decrease in alpha-actinin-4 expression reduced the invasion potential. These results indicated that the overexpression of alpha-actinin-4 was associated with an aggressive phenotype of OSCC. The study indicated that alpha-actinin-4 could be a potential molecular target for gene therapy by RNAi targeting for OSCC.
Keywords: α-actinin-4 / invasion / metastasis / oral squamous cell carcinoma / RNA interference
URI: http://hdl.handle.net/10069/22538
ISSN: 09015027
DOI: 10.1016/j.ijom.2009.10.003
PubMed ID: 19913389
Rights: Copyright © 2009 International Association of Oral and Maxillofacial Surgeons Published by Elsevier Ltd.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22538

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