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Bactericidal Activity in Filtrated Supernatant of Streptococcus Sanguinis against Multidrug-Resistant Pseudomonas Aeruginosa


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タイトル: Bactericidal Activity in Filtrated Supernatant of Streptococcus Sanguinis against Multidrug-Resistant Pseudomonas Aeruginosa
著者: Watanabe, Kiwao / Senba, Masachika / Ichinose, Akitoyo / Yamamoto, Takeshi / Ariyoshi, Koya / Matsumoto, Keizo
発行日: 2009年10月
出版者: Tohoku University Medical Press
引用: The Tohoku Journal of Experimental Medicine, 219(2), pp.79-84; 2009
抄録: In the past decade, multidrug-resistant Pseudomonas aeruginosa (MDRP) infection has become a serious clinical problem, due to the limitation of drug choices to fight against the bacteria. Here we explored the bactericidal activity in the filtrated supernatant of Streptococcus (S.) sanguinis against Pseudomonas (P.) aeruginosa. S. sanguinis is one of the α-hemolytic streptococci that commonly reside in the human oral cavity. A strain of S. sanguinis, isolated from the sputum of a pulmonary-disease patient, was cultured for overnight. The filtered supernatant was tested for bactericidal effect using the minimum bactericidal concentration method on 20 strains of P. aeruginosa, including two MDRP and five mucoid-type strains. The viable number of P. aeruginosa was decreased with time after exposing to the filtrated supernatant of S. sanguinis, and collapsed bacteria were detected with electron microscopy. Of the 20 strains, 19 (95%) strains of P. aeruginosa were affected by bactericidal effect. Among other species of bacteria examined, the filtrated supernatant of S. sanguinis showed remarkable bactericidal effect on 49% of indole-positive Proteus species (4/9 strains) and 60% of Acinetobacter (A.) baumannii (6/10 strains). We next investigated the property of bactericidal activity in filtrated supernatant by treating with proteinase K or autoclave. There was no change in the bactericidal activity of the filtrated supernatant after each treatment, excluding the involvement of protein and plasmid. Here, we identify the bactericidal activity in the filtrated supernatant of S. sanguinis against MDRP. This unexpected observation may contribute to the development of a novel therapeutic drug against P. aeruginosa.
キーワード: Acinetobacter baumannii / Bactericidal effect / Multidrug-resistant Pseudomonas aeruginosa / Pseudomonas aeruginosa / Streptococcus sanguinis
URI: http://hdl.handle.net/10069/22645
ISSN: 00408727
DOI: 10.1620/tjem.219.79
権利: Copyright (c) 2009 Tohoku University Medical Press
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:120 学術雑誌論文

引用URI : http://hdl.handle.net/10069/22645

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