DSpace university logo mark
詳細検索
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > 110 医歯薬学総合研究科 > 110 学術雑誌論文 >

Highly stomach-selective gene transfer following gastric serosal surface instillation of naked plasmid DNA in rats.


ファイル 記述 サイズフォーマット
JouGas43_912.pdf638.12 kBAdobe PDF本文ファイル

タイトル: Highly stomach-selective gene transfer following gastric serosal surface instillation of naked plasmid DNA in rats.
著者: Nishi, Junya / Fumoto, Shintaro / Ishii, Hiroki / Kodama, Yukinobu / Nakashima, Mikiro / Sasaki, Hitoshi / Nakamura, Junzo / Nishida, Koyo
発行日: 2008年12月
出版者: Springer Japan
引用: Journal of gastroenterology, 43(12), pp.912-919; 2008
抄録: BACKGROUND: The purpose of this study was to achieve stomach-selective gene transfer in rats by our simple and novel administration method, which is gastric serosal surface instillation of naked plasmid DNA (pDNA). METHODS: Naked pDNA encoding firefly luciferase as a reporter gene was instilled onto the gastric serosal surface in male Wistar rats. As controls, we performed intraperitoneal, intragastric and intravenous administration of naked pDNA. At appropriate time intervals, we measured luciferase activities in the stomach and other tissues. RESULTS: Gene expression in the stomach 6 h after gastric serosal surface instillation of naked pDNA (5 microg) was significantly higher than that after using other administration methods. The present study is the first report on stomach-selective gene transfer following instillation of naked pDNA onto the gastric serosal surface in rats. Also, the gene expression level in the stomach 6 h after gastric serosal surface instillation of naked pDNA was markedly higher than that in other tissues. In a dose-dependent study, the gene expression level was saturated over 5 microg. Gene expression in the stomach was detected 3 h after gastric serosal surface instillation of naked pDNA. The gene expression level peaked 12-24 h after instillation of naked pDNA, then decreased to a level similar to 3 h at 48 h. CONCLUSIONS: Gastric serosal surface in stillation of naked pDNA can be a highly stomach-selective gene transfer method in rats.
キーワード: Gene delivery / Naked plasmid DNA / Rat / Stomach / Targeting
URI: http://hdl.handle.net/10069/22669
ISSN: 09441174
DOI: 10.1007/s00535-008-2301-7
PubMed ID: 19107334
権利: © Springer Japan 2008. / The original publication is available at www.springerlink.com
資料タイプ: Journal Article
原稿種類: author
出現コレクション:110 学術雑誌論文

引用URI : http://hdl.handle.net/10069/22669

このリポジトリに保管されている文献はすべて著作権により保護されています。
印刷やダウンロード等データの複製は、調査研究・教育または学習を目的とする場合に限定されます。

 

Valid XHTML 1.0! Copyright © 2006-2015 長崎大学附属図書館 - お問い合わせ Powerd by DSpace