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Diverse Effects of FK506 on the Apoptosis of Hepatocytes and Infiltrating Lymphocytes in an Allografted Rat Liver.


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Title: Diverse Effects of FK506 on the Apoptosis of Hepatocytes and Infiltrating Lymphocytes in an Allografted Rat Liver.
Authors: Moriuchi, Hiroki / Kamohara, Yukio / Eguchi, Susumu / Gu, Weili / Fujioka, Hikaru / Yamamoto, Takao / Tajima, Yoshitsugu / Kanematsu, Takashi / Koji, Takehiko
Issue Date: 1-May-2011
Publisher: Elsevier Inc.
Citation: The Journal of surgical research, 167(1), pp.131-139; 2011
Abstract: BACKGROUND: The current study investigated whether FK506 (FK) regulates the apoptotic systems in allografted rat liver and the contribution of Fas/Fas-ligand system and Bcl-2 family during acute rejection. MATERIALS AND METHODS: The recipients were divided into three groups, the allo, the allo-FK, and the syn group. Rats were euthanized 1, 3, 5, and 7 d after OLT. Apoptotic activity was explored using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. The expression of Fas/Fas-ligand and Bcl-2/Bax in the grafted livers was investigated by Western blotting and immunohistochemistry. RESULTS: The apoptotic index (AI) of hepatocytes in the allo-FK group was less than that in the allo group. Fas in the allo group was more intense than that in the allo-FK group in the periportal areas on day 1 and 3, while Bcl-2 in the allo group was less intense than that in the allo-FK group in the pericaval areas at all time-points after OLT. CONCLUSION: FK provides beneficial antiapoptotic effects on hepatocytes in the grafted rat livers through both the down-regulation of Fas expression in the periportal areas and the up-regulation of Bcl-2 expression in the pericaval areas.
Keywords: apoptosis / Bax / Bcl-2 / Fas / Fas ligand / FK506 / liver transplantation
URI: http://hdl.handle.net/10069/22758
ISSN: 00224804
DOI: 10.1016/j.jss.2009.06.054
PubMed ID: 20018304
Rights: Copyright © 2009 Elsevier Inc. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22758

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