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Multiplex real-time polymerase chain reaction for rapid detection of beta-lactamase-negative, ampicillin-resistant Haemophilus influenzae.


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Title: Multiplex real-time polymerase chain reaction for rapid detection of beta-lactamase-negative, ampicillin-resistant Haemophilus influenzae.
Authors: Nakamura, Shigeki / Yanagihara, Katsunori / Morinaga, Yoshitomo / Izumikawa, Koichi / Seki, Masafumi / Kakeya, Hiroshi / Yamamoto, Yoshihiro / Kamihira, Shimeru / Kohno, Shigeru
Issue Date: May-2009
Publisher: Elsevier Inc.
Citation: Diagnostic microbiology and infectious disease, 64(1), pp.64-69; 2009
Abstract: We evaluated a multiplex real-time quantitative polymerase chain reaction (PCR) method for quantification of Haemophilus influenzae and rapid detection of beta-lactam-resistant strains. We designed 5 PCR primer sets to simultaneously detect the beta-lactam-resistant genes and quantify the pathogen. To demonstrate the validity of this assay, we used 191 clinical isolates, including 141 H. influenzae strains, and 100 purulent sputum samples, including 30 samples from which H. influenzae had been isolated. This assay showed 92.9% sensitivity and 91.8% specificity for detecting beta-lactam-resistant genes, relative to the conventional phenotypic method, and this assay correlated well with conventional quantitative culture counts. By using this assay, we could quantify H. influenzae and identify beta-lactam susceptibility in only 3 h and with only one tube. This method will be helpful for the rapid detection of H. influenzae infections and the selection of appropriate antibiotics.
Keywords: BLNAR / Multiplex real-time PCR / Rapid detection
URI: http://hdl.handle.net/10069/22762
ISSN: 07328893
DOI: 10.1016/j.diagmicrobio.2009.01.023
PubMed ID: 19232868
Rights: Copyright © 2009 Elsevier Inc. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22762

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