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Sensitive and Real-Time Method for Evaluating Corneal Barrier Considering Tear Flow

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Title: Sensitive and Real-Time Method for Evaluating Corneal Barrier Considering Tear Flow
Authors: Nakamura, Tadahiro / Teshima, Mugen / Kitahara, Takashi / Sasaki, Hitoshi / Uematsu, Masafumi / Kitaoka, Takashi / Nakashima, Mikiro / Nishida, Koyo / Nakamura, Junzo / Higuchi, Shun
Issue Date: Jan-2010
Publisher: The Pharmaceutical Society of Japan
Citation: Biological & Pharmaceutical Bulletin, 33(1), pp.107-110; 2010
Abstract: We developed a new electrophysiological method mimicking tear flow to evaluate the epithelial tight junction of rabbit cornea quantitatively. We investigated the effect of tear flow on the corneal damage induced by ophthalmic preservatives using this method. An Ussing chamber system with Ag/AgCl electrodes was used in the electrophysiological experiment. The excised rabbit cornea was mounted in the Ussing chamber and the precorneal solution in the chamber was perfused with a peristaltic pump at the rate of human tear flow. Corneal transepithelial electrical resistance (TEER) was monitored as corneal barrier ability. In the electrophysiological method mimicking tear flow, we observed stable TEER, which rapidly decreased with benzalkonium chloride (BAC), an eye drop preservative. Using this system, we first found that 0.004% BAC decreased corneal TEER reversibly. A high concentration of BAC showed strong irreversible damage to the tight junction. The influence of BAC on corneal TEER was not only concentration-dependent but also tear flow rate-dependent. The electrophysiological method mimicking tear flow was useful to evaluate the corneal barrier quantitatively. Using this method, we clarified that the tear flow was important to protect the corneal damage induced by preservatives.
Keywords: Benzalkonium chloride / Corneal barrier / Corneal transepithelial electrical resistance / Electrophysiological method / Mimicking tear flow / Ussing chamber
URI: http://hdl.handle.net/10069/22958
ISSN: 09186158
DOI: 10.1248/bpb.33.107
Rights: © 2010 Pharmaceutical Society of Japan.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/22958

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