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Enhanced osseointegration by the chemotactic activity of plasma fibronectin for cellular fibronectin positive cells.

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タイトル: Enhanced osseointegration by the chemotactic activity of plasma fibronectin for cellular fibronectin positive cells.
著者: Jimbo, Ryo / Sawase, Takashi / Shibata, Yasuaki / Hirata, Kazunari / Hishikawa, Yoshitaka / Tanaka, Yasuhiro / Bessho, Kazuhisa / Ikeda, Tohru / Atsuta, Mitsuru
発行日: 2007年 8月
出版者: Elsevier Ltd.
引用: Biomaterials, 28(24), pp.3469-3477; 2007
抄録: Plasma fibronectin (pFN) is known to regulate cell growth, differentiation or survival of osteoblasts in vitro. It is also speculated to be important for the early phase of osseointegration, however, its actual in vivo behavior is unknown. The objective of this study is to clarify the role of pFN during osseointegration. We developed a titanium ion-plated acrylic implant (Ti-acryl) for thin sectioning without removal of the implant. Either Ti-acryl or pFN-coated Ti-acryl (FN-Ti-acryl) was implanted in the mouse femur. Samples were taken on days 1-7 and on day 14 after the operation, and were decalcified and paraffin embedded. The bone healing process and immunofluorescence localization of pFN and cellular fibronectin (cFN), a marker for fibroblastic cells were examined. Simultaneously, the effect of pFN on chemotaxis, proliferation and differentiation of bone marrow stromal cells (BMSCs) was analyzed in vitro. The in vivo results showed that faster direct bone formation was seen for the FN-Ti-acryl group compared to the Ti-acryl group. The in vitro results showed that pFN significantly promoted BMSCs chemotaxis, however, had no effect on proliferation or differentiation. The results indicate that pFN regulated chemotaxis of osteogenic cells and coating the implant with pFN enhanced earlier osseointegration.
キーワード: Chemotaxis / Fibronectin / Histomorphometry / Implant / Osseointegration
URI: http://hdl.handle.net/10069/23017
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2007.04.029
PubMed ID: 17512051
権利: Copyright © 2007 Elsevier Ltd All rights reserved.
資料タイプ: Journal Article
原稿種類: author
出現コレクション:110 学術雑誌論文

引用URI : http://hdl.handle.net/10069/23017



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