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CEL-I, an N-acetylgalactosamine (GalNAc)-specific C-type lectin, induces nitric oxide production in RAW264.7 mouse macrophage cell line.

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タイトル: CEL-I, an N-acetylgalactosamine (GalNAc)-specific C-type lectin, induces nitric oxide production in RAW264.7 mouse macrophage cell line.
著者: Yamanishi, Tomohiro / Hatakeyama, Tomomitsu / Yamaguchi, Kenichi / Oda, Tatsuya
発行日: 2009年 8月
出版者: Japanese Biochemical Society / 日本生化学会
引用: Journal of biochemistry, 146(2), pp.209-217; 2009
抄録: We found that CEL-I, a GalNAc-specific C-type lectin isolated from the marine invertebrate Holothuroidea (Cucumaria echinata), induces inducible nitric oxide synthase (iNOS) expression and NO production in RAW264.7 cells. The NO production was inhibited by an iNOS inhibitor, L-NAME, but was not by a lipopolysaccharide (LPS) inhibitor, polymyxin B. In the presence of 0.1-M GalNAc, increased NO production by CEL-I-treated RAW264.7 cells was observed rather than the inhibition. Bovine serum albumin (BSA) significantly inhibited the CEL-I-induced NO production as well as the binding of FITC-labelled CEL-I on RAW264.7 cells. Three MAP kinase inhibitors (specific to extra-cellular regulated kinase, c-jun NH(2)-terminal kinase and p38 MAP kinase) inhibited CEL-I-induced NO production with different extents. Heat-treatment of CEL-I resulted in a decreased activity of CEL-I depending on the temperature. These results suggest that CEL-I induces NO production in RAW264.7 cells through the protein-cell interaction rather than the binding to the specific carbohydrate chains on the cell surface.
キーワード: CEL-I / C-type lectin / Cucumaria echinata / inducible nitric oxide synthase (iNOS) / nitric oxide (NO)
URI: http://hdl.handle.net/10069/23065
ISSN: 0021924X
DOI: 10.1093/jb/mvp057
PubMed ID: 19351706
関連リンク : http://jb.oxfordjournals.org/
権利: © The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved / This is a pre-copy-editing, author-produced PDF of an article accepted for publication in "Journal of Biochemistry" following peer review. The definitive publisher-authenticated version "Journal of Biochemistry 2009 146(2):209-217" is available online at: http://jb.oxfordjournals.org/cgi/content/abstract/146/2/209
資料タイプ: Journal Article
原稿種類: author
出現コレクション:080 学術雑誌論文

引用URI : http://hdl.handle.net/10069/23065



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