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Possible involvement of hemolytic activity in the contact-dependent lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis


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Title: Possible involvement of hemolytic activity in the contact-dependent lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis
Authors: Zou, Yanan / Yamasaki, Yasuhiro / Matsuyama, Yukihiko / Yamaguchi, Kenichi / Honjo, Tsuneo / Oda, Tatsuya
Issue Date: May-2010
Publisher: Elsevier B.V.
Citation: Harmful Algae, 9(4), pp.367-373; 2010
Abstract: We investigated the effects of two strains (SUO-1 and FUK) of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis. The SUO-1 strain was highly toxic to rotifers, whereas the FUK strain was less toxic. After 10-h incubations, the survivorship of rotifers exposed to SUO-1 and FUK was 20% and 95%, respectively. Both the cell-free culture supernatant and the ruptured cell suspension prepared from these strains were not toxic to rotifers. Furthermore, when direct contact between K. mikimotoi and rotifers was interrupted with a cell-impermeable membrane (3-μm pores), the toxicity of both the SUO-1 and FUK strains of K. mikimotoi to rotifers were completely inhibited even after a 24-h exposure. Cell suspensions of SUO-1 showed hemolytic activity toward horse erythrocytes, but the FUK strain did not. The cell-free supernatant and the ruptured cell suspension of SUO-1 showed no significant hemolytic activity. These results suggest that this highly toxic strain of K. mikimotoi causes lethality in rotifers by direct contact in which live cell-mediated hemolytic activity might be a contributing factor.
Keywords: Brachionus plicatilis / Cell contact / Hemolytic activity / Karenia mikimotoi / Reactive oxygen species / Toxic effect
URI: http://hdl.handle.net/10069/23139
ISSN: 15689883
DOI: 10.1016/j.hal.2010.01.005
Rights: Copyright © 2010 Elsevier B.V. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/23139

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