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Evaluation of chemiluminescence reagents for selective detection of reactive oxygen species.

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Title: Evaluation of chemiluminescence reagents for selective detection of reactive oxygen species.
Authors: Yamaguchi, Shinya / Kishikawa, Naoya / Ohyama, Kaname / Ohba, Yoshihito / Kohno, Maiko / Masuda, Toshinobu / Takadate, Akira / Nakashima, Kenichiro / Kuroda, Naotaka
Issue Date: 14-Apr-2010
Publisher: Elsevier B.V.
Citation: Analytica chimica acta, 665(1), pp.74-78; 2010
Abstract: In order to evaluate the chemiluminescence (CL) reagents for selective detection of reactive oxygen species (ROS), we comprehensively measured the CL responses of 20 CL reagents (three luminol derivatives, two imidazopyrazinone derivatives, eight lophine derivatives, six acridinium ester derivatives and lucigenin) against six types of ROS (superoxide anion: O(2)(*-), hydroxyl radical: *OH, hydrogen peroxide: H(2)O(2), hypochlorite anion: ClO(-), singlet oxygen: (1)O(2), and nitric oxide: NO). As a result of the screening, it was found that nine CL reagents selectively detected O(2)(*-) while one CL reagent selectively detected *OH. However, no CL reagent had selectivity on the detection of H(2)O(2), ClO(-), (1)O(2) and NO. Our screening results could help to select the most suitable CL reagent for selective determination of different ROS. As an application study, 4-methoxyphenyl-10-methylacridinium-9-carboxylate (MMAC), one of the acridinium ester derivatives, showed high selectivity on the detection of O(2)(*-), and thus was applied to the assay of superoxide dismutase (SOD) activity. The dynamic range and detection limit of the developed CL assay were 0.1-10 and 0.06 U mL(-1), respectively. Significant correlation (r=0.997) was observed between the results by the CL assay using MMAC and the spectrophotometric assay using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt.
Keywords: Acridinium ester / Chemiluminescent probe / Reactive oxygen species / Screening / Superoxide dismutase
URI: http://hdl.handle.net/10069/23180
ISSN: 00032670
DOI: 10.1016/j.aca.2010.03.025
PubMed ID: 20381693
Rights: Crown copyright © 2010 Published by Elsevier B.V.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/23180

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