DSpace university logo mark
詳細検索
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > 220 環東シナ海海洋環境資源研究センター > 220 報告書 > 220 研究報告書 >

Monitoring Bacterial Biodiversity in Surface Sediment Using Terminal Restriction Fragment Length Polymorphism Analysis (T-RFLP): Application to Coastal Environment


ファイル 記述 サイズフォーマット
nu2010151.pdf1.25 MBAdobe PDF本文ファイル

タイトル: Monitoring Bacterial Biodiversity in Surface Sediment Using Terminal Restriction Fragment Length Polymorphism Analysis (T-RFLP): Application to Coastal Environment
著者: Thiyagarajan, Vengatesen / Lau, Stanley / Tsoi, Mandy / Zhang, Wen / Qian, Pei Yuan
発行日: 2010年
出版者: TERRAPUB
引用: Coastal Environmental and Ecosystem Issues of the East China Sea ,pp.151-163; 2010
抄録: By using traditional bacterial culture methods, only less than 1% of bacteria from environmental samples can be cultivated for biodiversity and microbial ecology studies. In recent years, however, rapid development in molecular biology and ecology has enabled us to explore genetic diversity of bacteria in environmental samples using culture-independent approaches. For example, the recent emergence of (ribosomal DNA) terminal restriction fragment length polymorphism (T-RFLP) analysis has made it become feasible to investigate bacterial diversity more accurately, reliably, speedily and at cheaper cost in coastal sediment samples. This brief paper provides an overview of pros and cons of the molecular approach, T-RFLP, in analyzing the diversity of bacteria in surface sediments of contrasting coastal environments in sub-tropical region. We used the terminal restriction fragment length polymorphism (T-RFLP) analysis to track the changes of bacterial community compositions in surface sediments collected from five sites along a pollution gradient at six sampling occasions (2004 to 2006) in subtropical Hong Kong waters. Our application of this technique in coastal environments illustrates the feasibility and the power of this novel approach for environmental monitoring and ecosystem recovery studies.
記述: Nagasaki University Major Research Project: Restoration of Marine Environment and Resources in East Asia
キーワード: surface sediment / Victoria Harbour / sewage pollutionbacterial community / T-RFLP
URI: http://hdl.handle.net/10069/23507
ISBN: 9784887041516
権利: © by TERRAPUB and Nagasaki University, 2010.
資料タイプ: Research Paper
原稿種類: publisher
出現コレクション:220 研究報告書

引用URI : http://hdl.handle.net/10069/23507

このリポジトリに保管されている文献はすべて著作権により保護されています。
印刷やダウンロード等データの複製は、調査研究・教育または学習を目的とする場合に限定されます。

 

Valid XHTML 1.0! Copyright © 2006-2015 長崎大学附属図書館 - お問い合わせ Powerd by DSpace