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Possible Factors Responsible for the Fish-Killing Mechanisms of the Red Tide Phytoplankton, Chattonella marina and Cochlodinium polykrikoides


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Title: Possible Factors Responsible for the Fish-Killing Mechanisms of the Red Tide Phytoplankton, Chattonella marina and Cochlodinium polykrikoides
Authors: Kim, Daekyung / Oda, Tatsuya
Issue Date: 2010
Publisher: TERRAPUB
Citation: Coastal Environmental and Ecosystem Issues of the East China Sea ,pp.245-268; 2010
Abstract: Generation of reactive oxygen species (ROS) such as superoxide (O2.) and hydroxyl radical (・OH) by Chattonella marina could be confirmed by ESR spin trapping method. Several lines of evidence suggested that cell surface structure of C. marina, glycocalyx, has NADPH-dependent O2. generation system. Immunohistochemical analysis of gill lamellae from yellowtail exposed to C. marina using antiserum against crude glycocalyx demonstrated that there were the antigns recognaised by the antiserum on the surface of gill lamellae. The results suggest that continuous accumulation of discharged glycocalyx on the gill surface occurs during C. marina exposure, which may be responsible for the ROSmediated severe gill tissue damage leading to fish death. As compare to C. marina, the levels of O2. and H2O2 detected in Cochlodinium polykrikoides were trace levels. Furthermore, no significant increase in O2. generation by C. polykrikoides was observed in the presence of lectins or fish mucus prepared from skin and gill of yellowtail, whereas C. marina generated increased level of O2. responding to these stimuli. The cell-free aqueous extract prepared from C. polykrikoides showed toxic effect on HeLa cells, but the extract of C. marina had almost no effect. Furthermore, gradual accumulation of polysaccharides in the medium was observed during the culture of C. polykrikoides, and the medium gradually became viscous, but no such change was observed in the medium of C. marina. These results suggest that ichthyotoxic mechanisms of C. marina and C. polykrikoides are different. ROS may play a significant role in the fish-killing activity of C. marina, whereas in the case of C. polykrikoides, certain toxic substances or polysaccharides are mainly responsible for the toxicity rather than ROS.
Description: Nagasaki University Major Research Project: Restoration of Marine Environment and Resources in East Asia
Keywords: red tide / Chattonella marina / Cochlodinium polykrikoides / glycocalyx / reactive oxygen species (ROS) / cytotoxicity / mucus / toxin
URI: http://hdl.handle.net/10069/23515
ISBN: 9784887041516
Rights: © by TERRAPUB and Nagasaki University, 2010.
Type: Research Paper
Text Version: publisher
Appears in Collections:Research Paper

Citable URI : http://hdl.handle.net/10069/23515

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