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Protective effect of Sanguisorbae Radix against peroxynitrite-mediated renal injury


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Title: Protective effect of Sanguisorbae Radix against peroxynitrite-mediated renal injury
Authors: Chen, Cui Ping / Yokozawa, Takako / Sekiya, Michiko / Hattori, Masao / Tanaka, Takashi
Issue Date: 28-Feb-2001
Publisher: Medical and Pharmaceutical Society for WAKAN-YAKU / 和漢医薬学会
Citation: Journal of traditional medicines, 18(1), pp.1-7; 2001
Abstract: 3-Nitrotyrosine, an oxidative product of protein that is produced via peroxynitrite (ONOO^-) nitration, was detected by HPLC analysis in plasma obtained from rats injected with lipopolysaccharide (LPS) and subjected to renal ischemia followed by reperfusion (LPS+ischemia-reperfusion), but not in rats subjected to sham-treatment. Rats pretreated with Sanguisorbae Radix extract orally for 30 days before LPS+ischemia-reperfusion, had lower 3-nitrotyrosine levels than rats without the pretreatment. Plasma levels of urea nitrogen and creatinine, indicators of renal dysfunction, were markedly lower in the animals pretreated with Sanguisorbae Radix extract than in those without the pretreatment. In addition, DNA fragmentation in renal tissues was significantly inhibited by administration of Sanguisorbae Radix prior to LPS+ischemia-reperfusion. These results suggest that Sanguisorbae Radix extract ameliorates oxidative damage caused by ONOO^-. / パーオキシナイトライトは蛋白中のチロシンをニトロ化して3-ニトロチロシンを生成するが,この3-ニトロチロシンをHPLCで測定した結果,リポポリサッカライドと虚血-再灌流を施したラット血漿で検出され,偽処理した場合には検出されなかった。一方,リポポリサッカライドと虚血-再灌流を施す前に30日間地楡エキスを経口投与したラットでは,非投与群より低い3-ニトロチロシン値を示し,腎機能の指標の血漿尿素窒素,クレアチニンレベルも著しく低下していた。また腎組織中のDNA断片化も抑制され,地楡エキスがパーオキシナイトライトによる酸化的損傷を軽減することが推測された。
Keywords: Sanguisorbae Radix / peroxynitrite / 3-nitrotyrosine / DNA fragmentation / renal function / rat
URI: http://hdl.handle.net/10069/23536
ISSN: 13406302
Relational Links: http://ci.nii.ac.jp/naid/110001865632/
Rights: Medical and Pharmaceutical Society for WAKAN-YAKU / 本文データは学協会の許諾に基づきCiNiiから複製したものである
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/23536

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