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Immunohistochemical and Semiquantitative Immunoblot Analyses of Nm23-Hl and H2 Isoforms in Normal Human Tissues

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タイトル: Immunohistochemical and Semiquantitative Immunoblot Analyses of Nm23-Hl and H2 Isoforms in Normal Human Tissues
著者: Tsuchiya, Benio / Sato, Yuichi / Urano, Takeshi / Baba, Hideo / Shiku, Hiroshi / Kameya, Toru
発行日: 1998年
出版者: The Japan Society of Histochemistry and Cytochemistry / 日本組織細胞化学会
引用: Acta Histochemica et Cytochemica, 31(5), pp.411-418; 1998
抄録: The total amount of nm23 protein, the relative ratios of H1 and H2 isoforms (H2/H1) and the localization of these proteins in human normal tissues were studied by a semiquantitative immunoblot technique followed by densitometry and immunohistochemistry with monoclonal antibody against nm23 protein (Pan-242). All tissues contained both isoforms recognized as the 20.5kD H1 protein and 18.5kD H2 protein by immunoblotting. Nm23 protein was abundant in liver, kidney and adrenal gland tissue, and scarce in heart and muscle. H2 levels were always higher than H1, but the isoform ratios (H2/H1) were variable from tissue to tissue. Immunostaining revealed that nm23 protein was predominantly present in cytoplasm and the pattern of staining was homogeneous in parenchymal cells of the liver, pancreas and colonic mucosa and heterogeneous in gastric mucosa and kidney. These results demonstrated that the levels of nm23 protein and the H2/H1 ratios and distribution of isoforms were different in each tissue, and suggests that, when the alterations of nm23 gene expression in tumor tissues are examined, the levels and ratios in non-tumorous tissues surrounding the tumor nest should be considered.
キーワード: nm23-H1 / nm23-H2 / Immunoblotting / Immunohistochemistry / AMeX-fixed tissue
URI: http://hdl.handle.net/10069/23712
ISSN: 00445991
権利: Copyright ©1998 By the Japan Society of Histochemistry and Cytochemistry
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:030 学術雑誌論文

引用URI : http://hdl.handle.net/10069/23712



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