DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > School of Medicine > Articles in academic journal >

Expression of keratinocyte growth factor and its receptor in rat tracheal cartilage: possible involvement in wound healing of the damaged cartilage.


File Description SizeFormat
AHC43_89.pdf1.34 MBAdobe PDFView/Open

Title: Expression of keratinocyte growth factor and its receptor in rat tracheal cartilage: possible involvement in wound healing of the damaged cartilage.
Authors: Abo, Takafumi / Nagayasu, Takeshi / Hishikawa, Yoshitaka / Tagawa, Tsutomu / Nanashima, Atsushi / Yamayoshi, Takatomo / Matsumoto, Keitaro / An, Shucai / Koji, Takehiko
Issue Date: 28-Jun-2010
Publisher: The Japan Society of Histochemistry and Cytochemistry / 日本組織細胞化学会
Citation: Acta Histochemica et Cytochemica, 43(3), pp.89-98; 2010
Abstract: Keratinocyte growth factor (KGF) is involved in the development and regeneration of a variety of tissues. To clarify the role of KGF in cartilage wound healing, we examined the expression of KGF and its receptor (KGFR) immunohistochemically in the wound healing area of rat tracheal cartilage, and the direct effect of recombinant KGF on the proliferation and differentiation of primary cultures of rat chondrocytes. KGF was found in the cytoplasm of both chondrocytes and perichondrial cells. On the other hand, KGFR was detected only in the plasma membrane of chondrocytes. Although the expression of KGF was similar in the cartilage and perichondrial area before and after injury, KGFR expression was induced after injury and limited to proliferating chondrocytes. The staining pattern of KGF and KGFR was same in the mature and the immature rat tracheal cartilage. Moreover, in vitro experiments using primary cultured chondrocytes revealed that KGF at 200 ng/ml significantly increased the number of chondrocytes (~1.5-fold), and significantly reduced acid mucopolysaccharide production. These results indicate that KGF stimulates chondrocyte proliferation, suggesting that KGF could therapeutically modulate the wound healing process in the tracheal cartilage.
Keywords: keratinocyte growth factor / tracheal cartilage / wound repair
URI: http://hdl.handle.net/10069/23715
ISSN: 00445991
DOI: 10.1267/ahc.10006
PubMed ID: 20628626
Rights: Copyright ©2010 By the Japan Society of Histochemistry and Cytochemistry
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/23715

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace