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Axenic culture of Brachionus plicatilis using antibiotics

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タイトル: Axenic culture of Brachionus plicatilis using antibiotics
著者: Suga, Koushirou / Tanaka, Yukari / Sakakura, Yoshitaka / Hagiwara, Atsushi
発行日: 2011年 3月
出版者: Springer
引用: Hydrobiologia, 662(1), pp.113-119; 2011
抄録: The rotifer Brachionus plicatilis culture is composed of complex microcosms including bacteria, protozoans, algae, and fungi. Previous studies reported methods to establish axenic rotifer cultures, but further refinement of these techniques is needed, for molecular biological research which requires pure culture to isolate nucleic acids from rotifers only. In order to render rotifer culture axenic, we tested five antibiotics: ampicillin (Amp), chloramphenicol (Cp), kanamycin (Km), nalidixic acid (Na), and streptomycin (Sm) at 30-100 μg/ml. Except for Cp, which reduces rotifer reproduction, all other antibiotics at the tested concentrations did not affect rotifer reproduction or show any toxic effects. A rotifer disinfection method was finally established by treating the resting eggs with 0.25% (w/v) sodium hypochlorite (NaOCl) for 3 min, washing with sterilized sea water, and then exposing the neonates to an Amp, Km, Na, and Sm mixture. Using four nutrient media, we confirmed that this protocol renders the rotifer culture bacterial and fungus free. The axenic rotifer culture generated here is useful not only for genetic analysis of Brachionus plicatilis, but for studying the rotifer life cycle without bacterial influence.
キーワード: Antibiotic / Bacteria free / Brachionus plicatilis / Resting egg disinfection
URI: http://hdl.handle.net/10069/24463
ISSN: 00188158
DOI: 10.1007/s10750-010-0488-0
権利: © Springer Science+Business Media B.V. 2010 / The original publication is available at www.springerlink.com
資料タイプ: Journal Article
原稿種類: author
出現コレクション:080 学術雑誌論文

引用URI : http://hdl.handle.net/10069/24463



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