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A multiplex PCR-based molecular identification of five morphologically related, medically important subgenus stegomyia mosquitoes from the genus Aedes (Diptera: Culicidae) found in the Ryukyu Archipelago, Japan


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Title: A multiplex PCR-based molecular identification of five morphologically related, medically important subgenus stegomyia mosquitoes from the genus Aedes (Diptera: Culicidae) found in the Ryukyu Archipelago, Japan
Authors: Higa, Yukiko / Toma, Takako / Tsuda, Yoshio / Miyagi, Ichiro
Issue Date: Sep-2010
Publisher: National Institute of Infectious Diseases / 国立感染症研究所
Citation: Japanese Journal of Infectious Diseases, 63(5), pp.312-316; 2010
Abstract: Internal transcribed spacer regions of ribosomal DNA were sequenced, and new species-specific primers were designed to simplify the molecular identification of five morphologically related subgenus Stegomyia mosquito species-Aedes aegypti, Ae. albopictus, Ae. riversi, Ae. flavopictus, and Ae. daitensis-found in the Ryukyu Archipelago, Japan. Each newly designed primer was able to amplify a species-specific fragment with a different size. Conditions for multiplex PCR were optimized to identify all five species in a single PCR. This method is a convenient tool for entomological field surveys, particularly in arbovirus endemic/epidemic areas where some of these species coexist.
URI: http://hdl.handle.net/10069/24511
ISSN: 13446304
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/24511

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