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Dexamethasone-induced plasminogen activator inhibitor-1 expression in human primary bone marrow adipocytes


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Title: Dexamethasone-induced plasminogen activator inhibitor-1 expression in human primary bone marrow adipocytes
Authors: Hozumi, Akira / Osaki, Makoto / Sakamoto, Kazutaka / Goto, Hisataka / Fukushima, Tatsuya / Baba, Hideo / Shindo, Hiroyuki
Issue Date: 2010
Publisher: Biomedical Research Press
Citation: Biomedical Research, 31(5), pp.281-286; 2010
Abstract: Several studies have demonstrated the association of plasminogen activator inhibitor-1 (PAI-1) with osteonecrosis, but the underlying mechanism of osteonecrosis and its relationship with local PAI-1 is not clear. The objective of this study was to evaluate PAI-1 production by primary human bone marrow adipocytes and the effects of glucocorticoid administration. Bone marrow was obtained from 25 individuals during prosthetic insertion. Mature adipocytes were cultured for 24 h with or without dexamethasone. PAI-1, adiponectin, tumor necrosing factor-α (TNFα) expression were measured by latex photometric immunoassay or RT-PCR. Adiponectin, TNFα and PAI-1 were detected in all culture media. PAI-1 expression was signiicantly increased by treatment with 10-6 mol/L dexamethasone up to 24 h in protein and mRNA levels, while the levels of other adipokines did not change by dexamethasone. These results suggest that bone marrow adipocytes may play important roles for the development of glucocorticoid-induced osteonecrotic diseases by enhancing PAI-1 expression.
URI: http://hdl.handle.net/10069/24560
ISSN: 03886107
DOI: 10.2220/biomedres.31.281
Rights: Copyright (c) 2010 Biomedical Research Press
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/24560

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