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Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus).


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Title: Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus).
Authors: Wang, Yajun / Osatomi, Kiyoshi / Nagatomo, Yoshimi / Yoshida, Asami / Hara, Kenji
Issue Date: Apr-2011
Publisher: Elsevier Inc.
Citation: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 158(4), pp.289-296; 2011
Abstract: Manganese-superoxide dismutase (Mn-SOD) from Japanese flounder (Paralichthys olivaceus) hepatopancreas has been purified with high purification (781-fold) and recovery (10.8%). The molecular mass of the purified enzyme was estimated to be 26kDa by SDS-PAGE under reducing conditions. In activity staining by native-PAGE, the Japanese flounder Mn-SOD gave three active bands and exhibited KCN-insensitive activity. In addition, the electrophoretic mobility of this enzyme was observed to be faster than that of Japanese flounder Cu,Zn-SOD. On the other hand, the N-terminal amino acid sequence of this Mn-SOD was determined to be 16 amino acid residues, and the sequence showed high homology to other Mn-SODs but not Japanese flounder Cu,Zn-SOD. Analysis of nucleotide and deduced amino acid sequences revealed that the Mn-SOD cDNA consisted of a 64bp 5'-non-coding region, a 675bp open reading frame encoding 225 amino acids, and a 465bp 3'-non-coding region. The first 27 amino acids containing a mitochondria-targeting signal were highly conserved among other Mn-SODs.
Keywords: Amino acid sequence / CDNA sequence / Hepatopancreas / Japanese flounder / Manganese-superoxide dismutase / Mitochondria-targeting signal / Purification
URI: http://hdl.handle.net/10069/24898
ISSN: 10964959
DOI: 10.1016/j.cbpb.2010.12.007
PubMed ID: 21199678
Rights: Copyright © 2011 Elsevier Inc. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/24898

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