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Human bone marrow adipocytes support dexamethasone-induced osteoclast differentiation and function through RANKL expression


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タイトル: Human bone marrow adipocytes support dexamethasone-induced osteoclast differentiation and function through RANKL expression
著者: Goto, Hisataka / Osaki, Makoto / Fukushima, Tatsuya / Sakamoto, Kazutaka / Hozumi, Akira / Baba, Hideo / Shindo, Hiroyuki
発行日: 2011年 2月
出版者: Biomedical Research Foundation / バイオメディカルリサーチプレス
引用: Biomedical Research, 32(1), pp.37-44; 2011
抄録: The TNF-family molecule, Receptor Activator of Nuclear factor κ B Ligand (RANKL) is known as a key regulator for bone remodeling, and is essential for the development and activation of osteoclasts. In this study, we examined the regulation of RANKL in primary human bone marrow adipocytes and the relationship between bone marrow adipocytes and bone metabolism. RANKL expression and the RANKL/osteoprotegerin (OPG) mRNA ratio in marrow adipocytes increased following dexamethasone treatment. In co-cultures of human osteoclast precursors and bone marrow adipocytes with dexamethasone, osteoclast precursors differentiated to TRAP-positive multi-nuclear cells. Moreover, the ability of bone resorption was confirmed in co-culture in flasks coated with calcium phosphate film. Osteoclast precursor differentiation and bone resorption were blocked by RANKL antibody pretreatment. TRAP-positive multinuclear cells did not form in co-culture without cell-to-cell contact conditions. We conclude that primary human bone marrow adi-pocytes have the ability to promote osteoclast differentiation and activities, similar to osteoblasts and other RANKL-expressing cells.
URI: http://hdl.handle.net/10069/25146
ISSN: 03886107
DOI: 10.2220/biomedres.32.37
関連リンク : http://hdl.handle.net/10069/26698
権利: Copyright (c) 2011 Biomedical Research Press
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:030 学術雑誌論文

引用URI : http://hdl.handle.net/10069/25146

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