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Cloning and Characterization of the Antiviral Activity of Feline Tetherin/BST-2.

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タイトル: Cloning and Characterization of the Antiviral Activity of Feline Tetherin/BST-2.
著者: Fukuma, Aiko / Abe, Masumi / Morikawa, Yuko / Miyazawa, Takayuki / Yasuda, Jiro
発行日: 2011年 3月29日
出版者: Public Library of Science
引用: PloS one, 6(3), pp.e18247; 2011
抄録: Human Tetherin/BST-2 has recently been identified as a cellular antiviral factor that blocks the release of various enveloped viruses. In this study, we cloned a cDNA fragment encoding a feline homolog of Tetherin/BST-2 and characterized the protein product. The degree of amino acid sequence identity between human Tetherin/BST-2 and the feline homolog was 44.4%. Similar to human Tetherin/BST-2, the expression of feline Tetherin/BST-2 mRNA was inducible by type I interferon (IFN). Exogenous expression of feline Tetherin/BST-2 efficiently inhibited the release of feline endogenous retrovirus RD-114. The extracellular domain of feline Tetherin/BST-2 has two putative N-linked glycosylation sites, N79 and N119. Complete loss of N-linked glycosylation by introduction of mutations into both sites resulted in almost complete abolition of its antiviral activity. In addition, feline Tetherin/BST-2 was insensitive to antagonism by HIV-1 Vpu, although the antiviral activity of human Tetherin/BST-2 was antagonized by HIV-1 Vpu. Our data suggest that feline Tetherin/BST-2 functions as a part of IFN-induced innate immunity against virus infection and that the induction of feline Tetherin/BST-2 in vivo may be effective as a novel antiviral strategy for viral infection.
URI: http://hdl.handle.net/10069/25147
ISSN: 19326203
DOI: 10.1371/journal.pone.0018247
PubMed ID: 21479233
権利: © 2011 Fukuma et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:120 学術雑誌論文

引用URI : http://hdl.handle.net/10069/25147



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