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Title: 呼吸器感染症診療における迅速検査の現状と将来
Other Titles: Rapid and accurate detection of resistant bacteria for respiratory tract infection--present situation and future.
Authors: 柳原, 克紀 / 上平, 憲
Authors (alternative): Yanagihara, Katsunori / Kamihira, Shimeru
Issue Date: May-2010
Publisher: 日本臨床検査医学会 / Nihon Rinsho Byori Gakkai
Citation: 臨床病理, 58(5), pp.508-516; 2010
Abstract: Respiratory tract infection (RTI) is a common and potentially life-threatening illness that continues to be a major medical problem. The rapid detection system such as Gram staining or urinary antigen detection for respiratory infection is useful. However, the genetic diagnosis for resistant pathogens has been expected because they have been prevalent. We evaluated the RQ-mPCR (real-time quantitative PCR combined with multiplex PCR) assay for drug resistant S. pneumonia infection. The RQ-mPCR method developed here had high sensitivity and specificity for pneumococci and could detect drug resistance in both clinical S. pneumoniae strains and in sputum samples. Furthermore, the results can be obtained directly from clinical samples within 3h. This method may be helpful for the rapid screening of resistance in pneumococcal isolates, and should allow the administration of earlier, more focused and effective treatment of drug-resistant S. pneumonia. We also established the rapid quantitative detection of metallo-beta-lactamase-producing P. aeruginosa in clinical isolates and samples using real-time PCR targeting gyrB (identification of P. aeruginosa) and blaIMP (identification of metallo-beta-lactamase). The present PCR assay was thus easily and quickly performed, and accurately detected Pseudomonas aeruginosa and metallo-beta-lactamase, and it should be helpful in the diagnosis and control of nosocomial infection. We expect that the genetic diagnosis will be an adjunct to, or a replacement of, conventional culture methods and susceptibility tests.
Keywords: 呼吸器感染症 / 薬剤耐性菌 / 肺炎球菌 / 緑膿菌 / 遺伝子診断 / respiratory tract infection / resistant bacteria / Streptococcus pneumoniae / Pseudomonas aeruginosa / PCR
URI: http://hdl.handle.net/10069/25430
ISSN: 00471860
PubMed ID: 20560460
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/25430

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