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Dedifferentiation of human primary thyrocytes into multilineage progenitor cells without gene introduction.


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タイトル: Dedifferentiation of human primary thyrocytes into multilineage progenitor cells without gene introduction.
著者: Suzuki, Keiji / Mitsutake, Norisato / Saenko, Vladimir / Suzuki, Masatoshi / Matsuse, Michiko / Ohtsuru, Akira / Kumagai, Atsushi / Uga, Tatsuya / Yano, Hiroshi / Nagayama, Yuji / Yamashita, Shunichi
発行日: 2011年 4月27日
出版者: Public Library of Science
引用: PLoS One, 6(4), e19354; 2011
抄録: While identification and isolation of adult stem cells have potentially important implications, recent reports regarding dedifferentiation/reprogramming from differentiated cells have provided another clue to gain insight into source of tissue stem/progenitor cells. In this study, we developed a novel culture system to obtain dedifferentiated progenitor cells from normal human thyroid tissues. After enzymatic digestion, primary thyrocytes, expressing thyroglobulin, vimentin and cytokeratin-18, were cultured in a serum-free medium called SAGM. Although the vast majority of cells died, a small proportion (∼0.5%) survived and proliferated. During initial cell expansion, thyroglobulin/cytokeratin-18 expression was gradually declined in the proliferating cells. Moreover, sorted cells expressing thyroid peroxidase gave rise to proliferating clones in SAGM. These data suggest that those cells are derived from thyroid follicular cells or at least thyroid-committed cells. The SAGM-grown cells did not express any thyroid-specific genes. However, after four-week incubation with FBS and TSH, cytokeratin-18, thyroglobulin, TSH receptor, PAX8 and TTF1 expressions re-emerged. Moreover, surprisingly, the cells were capable of differentiating into neuronal or adipogenic lineage depending on differentiating conditions. In summary, we have developed a novel system to generate multilineage progenitor cells from normal human thyroid tissues. This seems to be achieved by dedifferentiation of thyroid follicular cells. The presently described culture system may be useful for regenerative medicine, but the primary importance will be as a tool to elucidate the mechanisms of thyroid diseases.
URI: http://hdl.handle.net/10069/25479
ISSN: 19326203
DOI: 10.1371/journal.pone.0019354
PubMed ID: 21556376
権利: © 2011 Suzuki et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:110 学術雑誌論文

引用URI : http://hdl.handle.net/10069/25479

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