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Dedifferentiation of human primary thyrocytes into multilineage progenitor cells without gene introduction.


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Title: Dedifferentiation of human primary thyrocytes into multilineage progenitor cells without gene introduction.
Authors: Suzuki, Keiji / Mitsutake, Norisato / Saenko, Vladimir / Suzuki, Masatoshi / Matsuse, Michiko / Ohtsuru, Akira / Kumagai, Atsushi / Uga, Tatsuya / Yano, Hiroshi / Nagayama, Yuji / Yamashita, Shunichi
Issue Date: 27-Apr-2011
Publisher: Public Library of Science
Citation: PLoS One, 6(4), e19354; 2011
Abstract: While identification and isolation of adult stem cells have potentially important implications, recent reports regarding dedifferentiation/reprogramming from differentiated cells have provided another clue to gain insight into source of tissue stem/progenitor cells. In this study, we developed a novel culture system to obtain dedifferentiated progenitor cells from normal human thyroid tissues. After enzymatic digestion, primary thyrocytes, expressing thyroglobulin, vimentin and cytokeratin-18, were cultured in a serum-free medium called SAGM. Although the vast majority of cells died, a small proportion (∼0.5%) survived and proliferated. During initial cell expansion, thyroglobulin/cytokeratin-18 expression was gradually declined in the proliferating cells. Moreover, sorted cells expressing thyroid peroxidase gave rise to proliferating clones in SAGM. These data suggest that those cells are derived from thyroid follicular cells or at least thyroid-committed cells. The SAGM-grown cells did not express any thyroid-specific genes. However, after four-week incubation with FBS and TSH, cytokeratin-18, thyroglobulin, TSH receptor, PAX8 and TTF1 expressions re-emerged. Moreover, surprisingly, the cells were capable of differentiating into neuronal or adipogenic lineage depending on differentiating conditions. In summary, we have developed a novel system to generate multilineage progenitor cells from normal human thyroid tissues. This seems to be achieved by dedifferentiation of thyroid follicular cells. The presently described culture system may be useful for regenerative medicine, but the primary importance will be as a tool to elucidate the mechanisms of thyroid diseases.
URI: http://hdl.handle.net/10069/25479
ISSN: 19326203
DOI: 10.1371/journal.pone.0019354
PubMed ID: 21556376
Rights: © 2011 Suzuki et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/25479

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