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Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.


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Title: Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.
Authors: Atarashi, Ryuichiro / Satoh, Katsuya / Sano, Kazunori / Fuse, Takayuki / Yamaguchi, Naohiro / Ishibashi, Daisuke / Matsubara, Takehiro / Nakagaki, Takehiro / Yamanaka, Hitoki / Shirabe, Susumu / Yamada, Masahito / Mizusawa, Hidehiro / Kitamoto, Tetsuyuki / Klug, Genevieve / McGlade, Amelia / Collins, Steven J / Nishida, Noriyuki
Issue Date: Feb-2011
Publisher: Nature Publishing Group
Citation: Nature Medicine, 17(2), pp.175-178; 2011
Abstract: The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions. Here we developed a new PrP(Sc) amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP(Sc) in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.
URI: http://hdl.handle.net/10069/25642
ISSN: 10788956
DOI: 10.1038/nm.2294
PubMed ID: 21278748
Rights: © 2011 Nature America, Inc. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/25642

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