DSpace university logo mark
詳細検索
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > 110 医歯薬学総合研究科 > 110 学術雑誌論文 >

Biofilm deficiency in polysaccharide intercellular adhesin-negative variants of staphylococcus epidermidis selected by subminimal inhibitory concentrations of gentamicin


ファイル 記述 サイズフォーマット
JID64_304.pdf238.09 kBAdobe PDF本文ファイル

タイトル: Biofilm deficiency in polysaccharide intercellular adhesin-negative variants of staphylococcus epidermidis selected by subminimal inhibitory concentrations of gentamicin
著者: Tamai, Takashi / Tsurumoto, Toshiyuki / Kajiyama, Shiro / Adachi, Shinji / Sakimura, Toshiyuki / Shindo, Hiroyuki
発行日: 2011年
出版者: National Institute of Infectious Diseases / 国立感染症研究所
引用: Japanese Journal of Infectious Diseases, 64(4), pp.304-308; 2011
抄録: Staphylococcus epidermidis is a cause of orthopedic device-related infection, and to treat such infection, biofilms should be controlled. Polysaccharide intercellular adhesin (PIA) is associated with the biofilm-forming ability of staphylococcal strains. PIA in biofilm-positive staphylococcal strains can be detected by the Congo red agar (CRA) method. In this study, we used the CRA method to examine the effects of subminimal inhibitory concentrations (sub-MICs) of 11 antibacterial agents on PIA production by S. epidermidis. We found that the PIA-negative variants were selected only by sub- MICs of gentamicin (GM). This PIA-negative phenotype was maintained over several generations in the absence of GM. Such selection occurred in six of eight clinical isolates, as well as in the biofilm-positive control strain. No such selection occurred with aminoglycoside antibiotics except for GM. Most of the PIA-negative variants that were selected by GM showed a markedly lower biofilm-forming ability on stainless steel washers than their untreated parent strains. In conclusion, variants with lower biofilmforming ability may be selected by a sub-MIC of GM. Investigation of the reason why variants with reduced biofilm-forming ability can be selected in the presence of sub-MICs of GM may contribute to strategies against biofilm-related infections.
URI: http://hdl.handle.net/10069/25859
ISSN: 13446304
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:110 学術雑誌論文

引用URI : http://hdl.handle.net/10069/25859

このリポジトリに保管されている文献はすべて著作権により保護されています。
印刷やダウンロード等データの複製は、調査研究・教育または学習を目的とする場合に限定されます。

 

Valid XHTML 1.0! Copyright © 2006-2015 長崎大学附属図書館 - お問い合わせ Powerd by DSpace