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Rubbing Gastric Serosal Surface Enhances Naked Plasmid DNA Transfer in Rats and Mice


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タイトル: Rubbing Gastric Serosal Surface Enhances Naked Plasmid DNA Transfer in Rats and Mice
著者: Mine, Toyoharu / Ishii, Hiroki / Nakajima, Sayuri / Yoshikawa, Naoki / Miyamoto, Hirotaka / Nakashima, Mikiro / Nakamura, Junzo / Fumoto, Shintaro / Nishida, Koyo
発行日: 2011年 9月 1日
出版者: The Pharmaceutical Society of Japan / 日本薬学会
引用: Biological & Pharmaceutical Bulletin, 34(9), pp.1514-1517; 2011
抄録: We have developed in vivo gene transfer to mesothelial cells on the peritoneal organs, including the stomach. Simple instillation of naked plasmid DNA onto the gastric serosal surface in mice resulted in effective but transient transgene expression. Here, we developed a simple method to improve not only the transfection efficiency but also the duration of transgene expression. Rubbing the gastric serosal surface using a medical spoon immediately after instillation of naked plasmid DNA onto the gastric serosal surface resulted in 59-fold higher transgene expression 24 h after administration in rats. Without rubbing, transgene expression decreased under the detection limit 7 d after administration. On the other hand, rubbing the gastric serosal surface with a medical spoon after instillation of plasmid DNA prolonged transgene expression for one month. Mechanistic study in mice revealed that improved transfection should not be due to stimulation of cell function such as macropinocytosis by rubbing because rubbing before instillation of plasmid DNA did not improve transfection. Plasmid DNA should enter effectively into cells during rubbing. These findings are valuable to develop an effective method of in vivo gene transfer into peritoneal organs.
キーワード: Gene therapy / Naked plasmid DNA / Non-viral gene delivery / Transfection
URI: http://hdl.handle.net/10069/26171
ISSN: 09186158
DOI: 10.1248/bpb.34.1514
権利: (c) 2011 The Pharmaceutical Society of Japan
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:050 学術雑誌論文

引用URI : http://hdl.handle.net/10069/26171

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