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Self-assemble gene delivery system for molecular targeting using nucleic acid aptamer.

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タイトル: Self-assemble gene delivery system for molecular targeting using nucleic acid aptamer.
著者: Kurosaki, Tomoaki / Higuchi, Norihide / Kawakami, Shigeru / Higuchi, Yuriko / Nakamura, Tadahiro / Kitahara, Takashi / Hashida, Mitsuru / Sasaki, Hitoshi
発行日: 2012年 1月10日
出版者: Elsevier BV
引用: Gene, 491(2), pp.205-209; 2012
抄録: We have developed a novel vector constructed with pDNA, polyethylenimine (PEI), and mucin 1 (MUC1) aptamer for tumor-targeted gene delivery. The MUC1 aptamer and non-specific aptamer were employed to coat the pDNA/PEI complexes electrostatically and stable nanoparticles were formed. The addition of a non-specific aptamer to the pDNA/PEI complex decreased gene expression in the human lung cancer cell line, A549 cells expressing MUC1 regularly. At the same time, the pDNA/PEI/MUC1 aptamer complex showed higher gene expression than pDNA/PEI/non-specific aptamer complex. Furthermore, the pDNA/PEI/MUC1 aptamer complex showed markedly high gene expression in tumor-bearing mice; thus, pDNA/PEI/MUC1 aptamer complexes are useful as a tumor-targeted gene delivery system with high transfection efficiency.
キーワード: Aptamer / Gene delivery / MUC1 / pDNA / Polyethylenimine
URI: http://hdl.handle.net/10069/26393
ISSN: 03781119
DOI: 10.1016/j.gene.2011.09.021
PubMed ID: 22001405
権利: Copyright © 2011 Published by Elsevier B.V.
資料タイプ: Journal Article
原稿種類: author
出現コレクション:130 学術雑誌論文

引用URI : http://hdl.handle.net/10069/26393



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