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Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients.

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Title: Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients.
Authors: Ahmed, Sameh / Kishikawa, Naoya / Ohyama, Kaname / Imazato, Takahiro / Ueki, Yukitaka / Kuroda, Naotaka
Issue Date: 15-Jul-2011
Publisher: Elsevier BV
Citation: Talanta, 85(1), pp.230-236; 2011
Abstract: Vitamin K is a fat-soluble vitamin involved in blood coagulation and bone metabolism. The detection and monitoring of vitamin K homologues in rheumatoid arthritis (RA) patients is a challenging problem due to the smaller concentrations of vitamin K and the presence of several interfering medications. Therefore, this study aimed to develop a new highly sensitive and selective chemiluminescence (CL) method designated to quantify vitamin K homologues in plasma of RA patients including phylloquinone (PK, vitamin K(1)), menaquinone-4 (MK-4, vitamin K(2)) and menaquinone-7 (MK-7, vitamin K(2)). The method was based on the unique photochemical properties of vitamin K homologues that were exploited for selective luminol CL reaction. The correlation coefficients of 0.998 or more were obtained in the concentration ranges of 0.1-100 ng mL(-1) vitamin K homologues. The detection limits were 0.03-0.1 ng mL(-1) in human plasma for vitamin K homologues. The developed HPLC-CL system was successfully applied for selective determination of vitamin K homologues in plasma of RA patients. The developed method may provide a useful tool for monitoring vitamin K homologues in different clinical studies such as RA, osteoporosis and hepatocellular carcinoma in which vitamin K is intervented.
Keywords: Luminol chemiluminescence / Photo-induced / Reactive oxygen species / Rheumatoid arthritis / Vitamin K homologues
URI: http://hdl.handle.net/10069/26411
ISSN: 00399140
DOI: 10.1016/j.talanta.2011.03.059
PubMed ID: 21645693
Rights: Copyright © 2011 Elsevier B.V. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/26411

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