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Epigallocatechin gallate suppresses peritoneal fibrosis in mice.

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Title: Epigallocatechin gallate suppresses peritoneal fibrosis in mice.
Authors: Kitamura, Mineaki / Nishino, Tomoya / Obata, Yoko / Furusu, Akira / Hishikawa, Yoshitaka / Koji, Takehiko / Kohno, Shigeru
Issue Date: 5-Jan-2012
Publisher: Elsevier Ireland Ltd.
Citation: Chemico-Biological Interactions, 195(1), pp.95-104; 2012
Abstract: Long-term peritoneal dialysis (PD) leads to histological changes in the peritoneal membrane. Angiogenesis and inflammation caused by glucose degradation products (GDPs) play crucial roles in peritoneal fibrosis. One such GDP is methylglyoxal (MGO), which enhances the formation of advanced glycation end products (AGEs). AGEs bind to their receptor (RAGE) and activate nuclear factor-κB (NF-κB), which is a key regulator of angiogenesis and inflammation. Recent studies have indicated that (-)-epigallocatechin gallate (EGCG), a tea polyphenol, inhibits angiogenesis and inflammation. Here, we examined whether EGCG suppresses peritoneal fibrosis in mice. Based on preliminary examination, 2mL of 40mM MGO or PD fluid was injected intraperitoneally and EGCG (50mg/kg) or saline was injected subcutaneously for 3weeks. In comparison to PD fluid+saline-treated mice, the peritoneal tissues of MGO+saline-treated mice showed marked thickening of the submesothelial compact zone. In the submesothelial compact zone of the MGO+saline-treated mice, CD31-positive vessels and vascular endothelial growth factor-positive cells were significantly increased, as were inflammation, F4/80-positive macrophages, and monocyte chemotactic protein-1. Moreover, 8-hydroxydeoxyguanosine, a marker of reactive oxygen species, and NF-κB, determined by Southwestern histochemistry, in the submesothelial compact zone were also increased in MGO+saline-treated mice. These changes were attenuated in MGO+EGCG-treated mice. We demonstrated that EGCG treatment suppresses peritoneal fibrosis via inhibition of NF-κB. Furthermore, EGCG inhibits reactive oxygen species production. The results of this study indicate that EGCG is a potentially novel candidate for the treatment of peritoneal fibrosis.
Keywords: (-)-Epigallocatechin gallate (EGCG) / Angiogenesis / Inflammation / Methylglyoxal (MGO) / Peritoneal dialysis (PD) / Peritoneal fibrosis
URI: http://hdl.handle.net/10069/27252
ISSN: 00092797
DOI: 10.1016/j.cbi.2011.11.002
PubMed ID: 22101032
Relational Links: http://hdl.handle.net/10069/32641
Rights: Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/27252

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