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Comparative study on modeccin- and phytohemagglutinin (PHA)-induced secretion of cytokines and nitric oxide (NO) in RAW264.7 cells.


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Title: Comparative study on modeccin- and phytohemagglutinin (PHA)-induced secretion of cytokines and nitric oxide (NO) in RAW264.7 cells.
Authors: Kim, Daekyung / Yamasaki, Yasuhiro / Jiang, Zedong / Nakayama, Yuki / Yamanishi, Tomohiro / Yamaguchi, Kenichi / Oda, Tatsuya
Issue Date: Jan-2011
Publisher: Institute of Biochemistry and Cell Biology
Citation: Acta biochimica et biophysica Sinica, 43(1), pp.52-60; 2011
Abstract: The effects of cytotoxic lectins, modeccin and phytohemagglutinin (PHA) on mouse macrophage cell line RAW264.7 was studied by detecting the induction of inflammatory mediators. Results showed that modeccin induced the release of tumor necrosis factor-α (TNF-α) from RAW264.7 cells with a bell-shape concentration-dependent profile. PHA that showed no significant cytotoxicity on RAW264.7 cells up to 100,000 ng/ml induced much higher level of TNF-α than modeccin. PHA simultaneously induced the secretion of granulocyte colony stimulation factor (G-CSF) from RAW264.7 cells with even much higher level than that of TNF-α, whereas modeccin did not. Furthermore, PHA induced the secretion of nitric oxide (NO) in RAW264.7 cells, while no significant level of NO was detected in the modeccin-treated cells. NH₄Cl (a lysomotoropic agent) and cycloheximide (a ribosome inhibitor) strongly inhibited modeccin-induced TNF-α secretion, but no significant inhibitory effects of these reagents on the PHA-induced TNF-α secretion were observed. Contrary to modeccin-induced TNF-α secretion, even slightly increased TNF-α secretion was observed in PHA-treated cells in the presence of 10 mM NH₄Cl. In addition, the inhibition profiles of modeccin-induced TNF-α secretion by various kinase inhibitors were different from those of PHA. These results suggested that the action mode of modeccin to stimulate RAW264.7 cells leading to the secretion of inflammatory molecules, including TNF-α, is distinct from that of PHA. On the other hand, significantly increased translocation of activator protein-1 (AP-1), a crucial transcription factor involved in expression of inflammatory molecules, into nucleus was observed in RAW264.7 cells treated with PHA and modeccin.
Keywords: G-CSF / modeccin / nitric oxide / PHA / TNF-
URI: http://hdl.handle.net/10069/27359
ISSN: 17457270
DOI: 10.1093/abbs/gmq105
PubMed ID: 21148191
Rights: © The Author 2010. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. / This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Acta biochimica et biophysica Sinica following peer review. The definitive publisher-authenticated version Acta biochimica et biophysica Sinica, 43(1), pp.52-60; 2011 is available online at: http://abbs.oxfordjournals.org/content/43/1/52.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/27359

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