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Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans.

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Title: Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans.
Authors: Hoshino, Tomonori / Kondo, Yoshio / Saito, Kan / Terao, Yutaka / Okahashi, Nobuo / Kawabata, Shigetada / Fujiwara, Taku
Issue Date: Sep-2011
Publisher: American Society for Microbiology.
Citation: Clinical and Vaccine Immunology, 18(9), pp.1552-1561; 2011
Abstract: In the development of a component vaccine against caries, the catalytic region (CAT) and glucan-binding domain (GBD) of glucosyltransferase B (GtfB) from Streptococcus mutans have been employed as target antigens. These regions were adopted as primary targets because they theoretically include epitopes associated with enzyme function. However, their antigenicities have not been fully evaluated. Although there are many reports about successful vaccination using these components, the principle has not yet been put to practical use. For these reasons, we came to doubt the effectiveness of the epitopes in vaccine production and reevaluated the antigenic region of GtfB by using in silico analyses combined with in vitro and in vivo experiments. The results suggested that the ca. 360-amino-acid variable region (VR) in the N terminus of GtfB is more reactive than CAT and GBD. This region is S. mutans and/or GtfB specific, nonconserved among other streptococcal Gtfs, and of unknown function. Immunization using an adenovirus vector-borne DNA vaccine confirmed that VR is an epitope that shows promise for the development of a caries vaccine.
Keywords: glucosyltransferase B / dental caries / antigenicity / DNA vaccine / adenovirus
URI: http://hdl.handle.net/10069/27399
ISSN: 15566811
DOI: 10.1128/CVI.05041-11
PubMed ID: 21795464
Rights: Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Type: Journal Article
Text Version: author
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/27399

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