DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > Faculty of Fisheries > Bulletin > Bulletin of the Faculty of Fisheries > No. 93 >

Cloning and nucleotide sequence analysis of the chloramphenicol and erythromycin resistance genes on a transferable R plasmid from the fish pathogen Photobacterium damselae subsp. piscicida


File Description SizeFormat
suisan93_41.pdf1.37 MBAdobe PDFView/Open

Title: Cloning and nucleotide sequence analysis of the chloramphenicol and erythromycin resistance genes on a transferable R plasmid from the fish pathogen Photobacterium damselae subsp. piscicida
Other Titles: 魚病原細菌Photobacterium damselae subsp. piscicidaの伝達性Rプラスミドのクロラムフェニコールおよび エリスロマイシン耐性因子のクローニングと塩基配列解析
Authors: Morii, Hideaki / Ishikawa, Yuka
Authors (alternative): 森井, 秀昭 / 石川, 由佳
Issue Date: Mar-2012
Publisher: 長崎大学水産学部 / The Faculty of Fisheries, Nagasaki University
Citation: 長崎大学水産学部研究報告, 93, pp.41-50; 2012
Abstract: Transferable resistance to various drugs was investigated in a strain of Photobacterium damselae subsp. piscicida from Japan. Drug resistance was transferred via three plasmids of 100, 50, and 40 kirobases (kb). Resistances to choloramphenicol and erythromycin were transferred on the 40-kb plasmid pPDP9106b. The cloned PstⅠ fragment of pPDP9106b was determined as being 6377 base pairs (bp). The chloramphenicol resistance gene conferring cross-resistance to florfenicol has been designated floR, which was found to be part of the 4279-bp transposon and coded 404 amino acid residues with a calculated molecular mass of 42.6 kDa. The transposon consisted of the gene floR, a putative regulatory gene (101 amino acid residues), and the transposase gene tnpA (497 amino acid residues) and constituted a circular form. The erythromycin resistance gene designated as ermM, which coded 303 amino acid residues with a calculated molecular mass of 35.6 kDa, was suggested to be a novel 23S rRNA methyltransferase from the presence of nine conserved sequence motifs that are important in target sequence, catalysis, and S-adenosyl-L-methionine binding and very low homology (35 to 15%) between the ErmM protein of P.damselae and the 23S rRNA methyltransferases of nineteen other bacteria.
Keywords: chloramphenicol resistance gene / cloning / erythromycin resistance gene / nucleotide sequence / Photobacterium damselae subsp. piscicida / transferable R plasmid
URI: http://hdl.handle.net/10069/28762
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:No. 93

Citable URI : http://hdl.handle.net/10069/28762

All items in NAOSITE are protected by copyright, with all rights reserved.

 

Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace