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Comparative study on antioxidative and macrophage-stimulating activities of polyguluronic acid (PG) and polymannuronic acid (PM) prepared from alginate


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タイトル: Comparative study on antioxidative and macrophage-stimulating activities of polyguluronic acid (PG) and polymannuronic acid (PM) prepared from alginate
著者: Ueno, Mikinori / Hiroki, Taichi / Takeshita, Satoshi / Jiang, Zedong / Kim, Daekyung / Yamaguchi, Kenichi / Oda, Tatsuya
発行日: 2012年 5月 1日
出版者: Elsevier Ltd.
引用: Carbohydrate Research, 352, pp.88-93; 2012
抄録: The antioxidant and macrophage-stimulating activities of polyguluronic acid (PG) and polymannuronic acid (PM) prepared from alginate were examined. A chemiluminescence (CL) method using a luminol analog, L-012, showed that both PM and PG scavenge superoxide produced by hypoxanthine–xanthine oxidase system in a concentration-dependent manner. At 100 μg/ml, PG showed slightly stronger superoxide scavenging activity than PM. In an electron spin resonance (ESR)-spin trapping method in which the Fenton reaction was used as hydroxyl radical generation system, we found that both PM and PG showed potent hydroxyl radical scavenging activity to a similar extent. Because PM and PG showed no chelating activity on Fe2+, it was confirmed that PM and PG can directly scavenge hydroxyl radical. No significant scavenging activity of PM and PG toward hydrogen peroxide was observed. Interestingly, the macrophage-stimulation activity of PG as measured by nitric oxide (NO)-production from mouse macrophage cell line RAW264.7 cells was evidently stronger than that of PM. Our results suggest that RAW264.7 cells might be able to distinguish the conformational differences between PM and PG, and respond differently to them, whereas the effects of such structural differences between PM and PG on the radical scavenging activities may not be so significant.
キーワード: Alginate oligomer / Polyguluronic acid / Polymannuronic acid / Antioxidative activity / Nitric oxide
URI: http://hdl.handle.net/10069/28904
ISSN: 00086215
DOI: 10.1016/j.carres.2012.02.005
権利: © Elsevier Ltd. / NOTICE: this is the author’s version of a work that was accepted for publication in Carbohydrate Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Carbohydrate Research, 352(2012)
資料タイプ: Journal Article
原稿種類: author
出現コレクション:080 学術雑誌論文

引用URI : http://hdl.handle.net/10069/28904

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