DSpace university logo mark
詳細検索
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > 130 病院 > 130 学術雑誌論文 >

Serum amyloid A triggers the mosodium urate -mediated mature interleukin-1β production from human synovial fibroblasts


ファイル 記述 サイズフォーマット
ART14_R119.pdf639.04 kBAdobe PDF本文ファイル

タイトル: Serum amyloid A triggers the mosodium urate -mediated mature interleukin-1β production from human synovial fibroblasts
著者: Migita, Kiyoshi / Koga, Tomohiro / Satomura, Kenshi / Izumi, Masahiro / Torigoshi, Takafumi / Maeda, Yumi / Izumi, Yasumori / Jiuchi, Yuka / Miyashita, Taiichiro / Yamasaki, Satoshi / Aiba, Yoshihiro / Komori, Atsumasa / Nakamura, Minoru / Motokawa, Satoru / Kawakami, Atsushi / Nakamura, Tadashi / Ishibashi, Hiromi
発行日: 2012年 5月18日
出版者: BioMed Central
引用: Arthritis Research & Therapy, 14(3), R119; 2012
抄録: Background: Monosodium urate (MSU) has been shown to promote inflammasome activation and interleukin-1β (IL-1β) secretion in monocyte/macrophages, but the cellular pathway and nod-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome activation in synovial tissues, remain elusive. In this study, we investigated the effects of MSU on synovial fibroblasts to elucidate the process of MSU-mediated synovial inflammation.Methods: Human synovial fibroblasts were stimulated with MSU in the presence or absence of serum amyloid A (SAA). The cellular supernatants were analyzed by immunoblotting using anti-IL-1β or anti-caspase-1 antibodies. IL-1β or NLRP3 mRNA expressions were analyzed by real-time PCR or reverse transcription-PCR (RT-PCR) method.Results: Neither SAA nor MSU stimulation resulted in IL-1β or interleukin-1α (IL-1α) secretions and pro-IL-1β processing in synovial fibroblasts. However, in SAA-primed synovial fibroblasts, MSU stimulation resulted in the activation of caspase-1 and production of active IL-1β and IL-1α. The effect of SAA on IL-1β induction was impaired in cells by silencing NLRP3 using siRNA or treating with caspase-1 inhibitor. In addition, SAA induced the secretion of cathepsin B and NLRP3 mRNA expression in synovial fibroblasts.Conclusions: Our data demonstrate that exposure of human synovial fibroblasts to SAA promotes MSU-mediated caspase-1 activation and IL-1β secretion in the absence of microbial stimulation. These findings provide insight into the molecular processes underlying the synovial inflammatory condition of gout.
キーワード: cathepsin B / cryopyrin / interleukin 1alpha / interleukin 1beta / messenger RNA / serum amyloid A / small interfering RNA / urate / article / controlled study / fibroblast / human / human cell / human tissue / immunoblotting / real time polymerase chain reaction / reverse transcription polymerase chain reaction / synovial fluid / synovitis
URI: http://hdl.handle.net/10069/29239
ISSN: 14786354
DOI: 10.1186/ar3849
権利: © 2012 Migita et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
資料タイプ: Journal Article
原稿種類: publisher
出現コレクション:130 学術雑誌論文

引用URI : http://hdl.handle.net/10069/29239

このリポジトリに保管されている文献はすべて著作権により保護されています。
印刷やダウンロード等データの複製は、調査研究・教育または学習を目的とする場合に限定されます。

 

Valid XHTML 1.0! Copyright © 2006-2015 長崎大学附属図書館 - お問い合わせ Powerd by DSpace