DSpace university logo mark
Advanced Search
Japanese | English 

NAOSITE : Nagasaki University's Academic Output SITE > University Hospital > Articles in academic journal >

Immunological milieu in the peritoneal cavity at laparotomy for gastric cancer

File Description SizeFormat
WJG18_1470.pdf890.74 kBAdobe PDFView/Open

Title: Immunological milieu in the peritoneal cavity at laparotomy for gastric cancer
Authors: Yoneda, Akira / Ito, Shinichiro / Susumu, Seiya / Matsuo, Mitsutoshi / Taniguchi, Ken / Tajima, Yoshitsugu / Eguchi, Susumu / Kanematsu, Takashi / Nagata, Yasuhiro
Issue Date: 7-Apr-2012
Publisher: WJG Press
Citation: World Journal of Gastroenterology, 18(13), pp.1470-1478; 2012
Abstract: AIM: To investigate the immunological repertoire in the peritoneal cavity of gastric cancer patients. METHODS: The peritoneal cavity is a compartment in which immunological host-tumor interactions can occur. However, the role of lymphocytes in the peritoneal cavity of gastric cancer patients is unclear. We observed 64 patients who underwent gastrectomy for gastric cancer and 11 patients who underwent laparoscopic cholecystectomy for gallstones and acted as controls. Lymphocytes isolated from both peripheral blood and peritoneal lavage were analyzed for surface markers of lymphocytes and their cytokine production by flow cytometry. CD4 +CD25 high T cells isolated from the patient's peripheral blood were co-cultivated for 4 d with the intra-peritoneal lymphocytes, and a cytokine assay was performed. RESULTS: At gastrectomy, CCR7 - CD45RA - CD8 + effector memory T cells were observed in the peritoneal cavity. The frequency of CD4 + CD25 high T cells in both the peripheral blood and peritoneal cavity was elevated in patients at advanced stage [control vs stage IV in the peripheral blood: 6.89 (3.39-10.4) vs 15.34 (11.37-19.31), P < 0.05, control vs stage IV in the peritoneal cavity: 8.65 (5.28-12.0) vs 19.56 (14.81-24.32), P < 0.05]. On the other hand, the suppression was restored with CD4 + CD25 high T cells from their own peripheral blood. This study is the first to analyze lymphocyte and cytokine production in the peritoneal cavity in patients with gastric cancer. Immune regulation at advanced stage is reversible at the point of gastrectomy. CONCLUSION: The immunological milieu in the peritoneal cavity of patients with advanced gastric cancer elicited a Th2 response even at gastrectomy, but this response was reversible.
Keywords: Cytokines / Gastric cancer / Lymphocytes / Peritoneal cavity / Regulatory T cell
URI: http://hdl.handle.net/10069/29378
ISSN: 10079327
DOI: 10.3748/wjg.v18.i13.1470
Rights: ©2012 Baishideng Publishing Group Co., Limited. All rights reserved. / © 1995-2012 Baishideng Publishing Group Co., Limited. Articles published by this Open-Access journal are distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/29378

All items in NAOSITE are protected by copyright, with all rights reserved.


Valid XHTML 1.0! Copyright © 2006-2015 Nagasaki University Library - Feedback Powerd by DSpace