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Pretreatment with Epidermal Growth Factor Enhances Naked Plasmid DNA Transfer onto Gastric Serosal Surface in Mice


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Title: Pretreatment with Epidermal Growth Factor Enhances Naked Plasmid DNA Transfer onto Gastric Serosal Surface in Mice
Authors: Miyamoto, Hirotaka / Baba, Sumie / Nakajima, Sayuri / Mine, Toyoharu / Yoshikawa, Naoki / Fumoto, Shintaro / Nishida, Koyo
Issue Date: 1-Jun-2012
Publisher: 日本薬学会 / The Pharmaceutical Society of Japan
Citation: Biological and Pharmaceutical Bulletin, 35(6), pp.903-908; 2012
Abstract: We have developed a simple administration method, which is gastric serosal surface instillation of naked plasmid DNA (pDNA) in experimental animals. The purpose of this study was to improve gastric gene transfer efficiency by pre-treatment with a macropinocytosis enhancer, such as fetuin or epidermal growth factor (EGF), in mice. A series of concentrations of fetuin were instilled onto gastric serosal surface prior to instillation of naked pDNA in mice; however, fetuin did not improve transgene expression in the stomach 6 h after administration of pDNA. EGF also did not affect transgene expression in the stomach when pDNA was instilled immediately after EGF instillation. On the other hand, when pDNA was instilled onto gastric serosal surface 24 h after EGF treatment, transgene expression in the stomach was significantly improved by 2.6-fold. In addition, transgene-positive cells were increased 5.3-fold by EGF pre-treatment. High transgene expression in the stomach lasted for 48 h in the EGF pre-treatment group in comparison with that in the no pre-treatment group. These findings are valuable to develop an effective method of in vivo gene transfer to the stomach.
Keywords: Epidermal growth factor / Gene therapy / Naked plasmid DNA / Non-viral gene delivery / Stomach
URI: http://hdl.handle.net/10069/29402
ISSN: 09186158
DOI: 10.1248/bpb.35.903
Rights: © 2012 The Pharmaceutical Society of Japan.
Type: Journal Article
Text Version: publisher
Appears in Collections:Articles in academic journal

Citable URI : http://hdl.handle.net/10069/29402

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