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Title: 海産ツボワムシ類2種の性フェロモン性状
Other Titles: Mate recognition pheromone of the marine rotifer Brachionus plicatilis and B, rotundiformis
Authors: 小谷, 知也 / 萩原, 篤志
Authors (alternative): Kotani, Tomonari / Hagiwara, Atsushi
Issue Date: Mar-2000
Publisher: 長崎大学水産学部 / 長崎大学水産学部研究報告, 81, pp.63-68; 2000
Citation: 長崎大学水産学部研究報告, 81: 63-68
Abstract: Mating behavior of male marine rotifer Brachionus is regulated by a female's mate recognition pheromone (MRP). It has already been clarified by the experiment using MRP of B. plicatilis that differences of MRP molecular structure among rotifer species and strains cause reproductive isolation. In this study, we tried isolating the MRP of B. rotundiformis and B. plicatilis, and compared the reactivity of both MRP to male's receptor. Using two kinds of lectin, MRPs of B. rotundiformis Koshiki strain and B. plicatilis Russian strain were isolated. Molecular weights of two-isolated MRP were determined with SDS-PAGE. To investigate the reactivity of MRP to male's receptor, four B. rotundiformis strains (Koshiki, Hamana, Thai, and Fiji) and three B. plicatilis strains were used. Molecular weight of both MRPs was 26 kDa. Russian MRP bound intensely with B. plicatilis male's receptor but not with B. rotundiformis. The reactivity of Koshiki MRP to control (Koshiki strain) was the same as to other six strains. Therefore, it is suggested that molecular structure of B. plicatilis MRP is different from that of B. rotundiformis although their molecular weights are the same.
Keywords: Brachionus plicatilis / B. rotundiformis / mate recognition pheromone / molecular weight / male receptor / molecular structure
URI: http://hdl.handle.net/10069/29718
ISSN: 05471427
Type: Departmental Bulletin Paper
Text Version: publisher
Appears in Collections:第81号

Citable URI : http://hdl.handle.net/10069/29718

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